2jt7
From Proteopedia
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|PDB= 2jt7 |SIZE=350|CAPTION= <scene name='initialview01'>2jt7</scene> | |PDB= 2jt7 |SIZE=350|CAPTION= <scene name='initialview01'>2jt7</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=DMY:DISTAMYCIN A'>DMY</scene> | + | |LIGAND= <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DMY:DISTAMYCIN+A'>DMY</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jt7 OCA], [http://www.ebi.ac.uk/pdbsum/2jt7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jt7 RCSB]</span> | ||
}} | }} | ||
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[[Category: Martino, L.]] | [[Category: Martino, L.]] | ||
[[Category: Virno, A.]] | [[Category: Virno, A.]] | ||
| - | [[Category: DMY]] | ||
[[Category: complex]] | [[Category: complex]] | ||
[[Category: distamycin some]] | [[Category: distamycin some]] | ||
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[[Category: tggggt]] | [[Category: tggggt]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:01:29 2008'' |
Revision as of 01:01, 31 March 2008
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| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR solution structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex
Overview
The complex between distamycin A and the parallel DNA quadruplex [d(TGGGGT)]4 has been studied by 1H NMR spectroscopy and isothermal titration calorimetry (ITC). To unambiguously assert that distamycin A interacts with the grooves of the quadruplex [d(TGGGGT)]4, we have analyzed the NMR titration profile of a modified quadruplex, namely [d(TGGMeGGT)]4, and we have applied the recently developed differential frequency-saturation transfer difference (DF-STD) method, for assessing the ligand-DNA binding mode. The three-dimensional structure of the 4:1 distamycin A/[d(TGGGGT)]4 complex has been determined by an in-depth NMR study followed by dynamics and mechanics calculations. All results unequivocally indicate that distamycin molecules interact with [d(TGGGGT)]4 in a 4:1 binding mode, with two antiparallel distamycin dimers that bind simultaneously two opposite grooves of the quadruplex. The affinity between distamycin A and [d(TGGGGT)]4 enhances ( approximately 10-fold) when the ratio of distamycin A to the quadruplex is increased. In this paper we report the first three-dimensional structure of a groove-binder molecule complexed to a DNA quadruplex structure.
About this Structure
2JT7 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structural and thermodynamic studies of the interaction of distamycin A with the parallel quadruplex structure [d(TGGGGT)]4., Martino L, Virno A, Pagano B, Virgilio A, Di Micco S, Galeone A, Giancola C, Bifulco G, Mayol L, Randazzo A, J Am Chem Soc. 2007 Dec 26;129(51):16048-56. Epub 2007 Dec 4. PMID:18052170
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Categories: Single protein | Martino, L. | Virno, A. | Complex | Distamycin some | Dna | Quadruplex | Telomere | Tggggt
