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2hw4

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Revision as of 08:35, 4 July 2018

Crystal structure of human phosphohistidine phosphatase

Structural highlights

2hw4 is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	PHPT1, PHP14 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PHP14_HUMAN] Exhibits phosphohistidine phosphatase activity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Phosphatases are a diverse group of enzymes that regulate numerous cellular processes. Much of what is known relates to the tyrosine, threonine, and serine phosphatases, whereas the histidine phosphatases have not been studied as much. The structure of phosphohistidine phosphatase (PHPT1), the first identified eukaryotic-protein histidine phosphatase, has been determined to a resolution of 1.9A using multiple-wavelength anomalous dispersion methods. This enzyme can dephosphorylate a variety of proteins (e.g. ATP-citrate lyase and the beta-subunit of G proteins). A putative active site has been identified by its electrostatic character, ion binding, and conserved protein residues. Histidine 53 is proposed to play a major role in histidine dephosphorylation based on these observations and previous mutational studies. Models of peptide binding are discussed to suggest possible mechanisms for substrate recognition.

First structure of a eukaryotic phosphohistidine phosphatase.,Busam RD, Thorsell AG, Flores A, Hammarstrom M, Persson C, Hallberg BM J Biol Chem. 2006 Nov 10;281(45):33830-4. Epub 2006 Sep 21. PMID:16990267^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Busam RD, Thorsell AG, Flores A, Hammarstrom M, Persson C, Hallberg BM. First structure of a eukaryotic phosphohistidine phosphatase. J Biol Chem. 2006 Nov 10;281(45):33830-4. Epub 2006 Sep 21. PMID:16990267 doi:10.1074/jbc.C600231200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hw4"

@@ Line 1: / Line 1: @@
 ==Crystal structure of human phosphohistidine phosphatase==
 <StructureSection load='2hw4' size='340' side='right' caption='[[2hw4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHPT1, PHP14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hw4 OCA], [http://pdbe.org/2hw4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hw4 RCSB], [http://www.ebi.ac.uk/pdbsum/2hw4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hw4 OCA], [http://pdbe.org/2hw4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2hw4 RCSB], [http://www.ebi.ac.uk/pdbsum/2hw4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2hw4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/2hw4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hw/2hw4_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hw4 ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">

2hw4

From Proteopedia

Revision as of 08:35, 4 July 2018

Crystal structure of human phosphohistidine phosphatase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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