2nuo
From Proteopedia
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|PDB= 2nuo |SIZE=350|CAPTION= <scene name='initialview01'>2nuo</scene>, resolution 1.50Å | |PDB= 2nuo |SIZE=350|CAPTION= <scene name='initialview01'>2nuo</scene>, resolution 1.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2gty|2gty]], [[2gux|2gux]], [[2gue|2gue]], [[2guc|2guc]], [[2gud|2gud]], [[2hyr|2hyr]], [[2hyq|2hyq]], [[2i43|2i43]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nuo OCA], [http://www.ebi.ac.uk/pdbsum/2nuo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nuo RCSB]</span> | ||
}} | }} | ||
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[[Category: Wlodawer, A.]] | [[Category: Wlodawer, A.]] | ||
[[Category: Ziolkowska, N E.]] | [[Category: Ziolkowska, N E.]] | ||
| - | [[Category: EDO]] | ||
| - | [[Category: GLC]] | ||
| - | [[Category: SO4]] | ||
[[Category: domain swapping]] | [[Category: domain swapping]] | ||
[[Category: griffithsin]] | [[Category: griffithsin]] | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:08:46 2008'' |
Revision as of 01:08, 31 March 2008
| |||||||
| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Related: | 2gty, 2gux, 2gue, 2guc, 2gud, 2hyr, 2hyq, 2i43
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a complex of griffithsin with glucose
Overview
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT) with glucose and N-acetylglucosamine were solved and refined at high resolution. In both complexes, all six monosaccharide-binding sites of GRFT were occupied and the mode of binding was similar to that of mannose. In our previous attempts to obtain a complex with N-acetylglucosamine by soaking, only a single site was occupied; thus, cocrystallization was clearly superior despite lower concentration of the ligand. Isothermal titration calorimetric experiments with N-acetylglucosamine, glucose, and mannose provided enthalpic evidence of distinct binding differences between the three monosaccharides. A comparison of the mode of binding of different monosaccharides is discussed in the context of the antiviral activity of GRFT, based on specific binding to high-mannose-containing complex carbohydrates found on viral envelopes.
About this Structure
2NUO is a Single protein structure of sequence from Griffithsia. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736
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