2nwm
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= Vinexin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= Vinexin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nwm OCA], [http://www.ebi.ac.uk/pdbsum/2nwm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nwm RCSB]</span> | ||
}} | }} | ||
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[[Category: vinexin sh3 domain]] | [[Category: vinexin sh3 domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:09:25 2008'' |
Revision as of 01:09, 31 March 2008
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| Gene: | Vinexin (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of the first SH3 domain of human Vinexin and its interaction with the peptides from Vinculin
Overview
Solution structure of the first Src homology (SH) 3 domain of human vinexin (V_SH3_1) was determined using nuclear magnetic resonance (NMR) method and revealed that it was a canonical SH3 domain, which has a typical beta-beta-beta-beta-alpha-beta fold. Using chemical shift perturbation and surface plasmon resonance experiments, we studied the binding properties of the SH3 domain with two different peptides from vinculin hinge regions: P856 and P868. The observations illustrated slightly different affinities of the two peptides binding to V_SH3_1. The interaction between P868 and V_SH3_1 belonged to intermediate exchange with a modest binding affinity, while the interaction between P856 and V_SH3_1 had a low binding affinity. The structure and ligand-binding interface of V_SH3_1 provide a structural basis for the further functional study of this important molecule.
About this Structure
2NWM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the first SH3 domain of human vinexin and its interaction with vinculin peptides., Zhang J, Li X, Yao B, Shen W, Sun H, Xu C, Wu J, Shi Y, Biochem Biophys Res Commun. 2007 Jun 15;357(4):931-7. Epub 2007 Apr 17. PMID:17467669
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