2nxx
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= Ultraspiracle (USP) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7070 Tribolium castaneum]), Ecdysone Receptor (EcR) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7070 Tribolium castaneum]) | |GENE= Ultraspiracle (USP) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7070 Tribolium castaneum]), Ecdysone Receptor (EcR) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7070 Tribolium castaneum]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2nxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nxx OCA], [http://www.ebi.ac.uk/pdbsum/2nxx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2nxx RCSB]</span> | ||
}} | }} | ||
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[[Category: Iwema, T.]] | [[Category: Iwema, T.]] | ||
[[Category: Moras, D.]] | [[Category: Moras, D.]] | ||
| - | [[Category: P1A]] | ||
[[Category: apo and holo ligand binding pocket]] | [[Category: apo and holo ligand binding pocket]] | ||
[[Category: hormone receptor]] | [[Category: hormone receptor]] | ||
[[Category: hormone/growth factor complex]] | [[Category: hormone/growth factor complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:09:59 2008'' |
Revision as of 01:10, 31 March 2008
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| , resolution 2.750Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | Ultraspiracle (USP) (Tribolium castaneum), Ecdysone Receptor (EcR) (Tribolium castaneum) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Ligand-Binding Domains of the T.castaneum (Coleoptera) Heterodimer EcrUSP Bound to Ponasterone A
Overview
Retinoid X receptor (RXR) and Ultraspiracle (USP) play a central role as ubiquitous heterodimerization partners of many nuclear receptors. While it has long been accepted that a wide range of ligands can activate vertebrate/mollusc RXRs, the existence and necessity of specific endogenous ligands activating RXR-USP in vivo is still matter of intense debate. Here we report the existence of a novel type of RXR-USP with a ligand-independent functional conformation. Our studies involved Tribolium USP (TcUSP) as representative of most arthropod RXR-USPs, with high sequence homology to vertebrate/mollusc RXRs. The crystal structure of the ligand-binding domain of TcUSP was solved in the context of the functional heterodimer with the ecdysone receptor (EcR). While EcR exhibits a canonical ligand-bound conformation, USP adopts an original apo structure. Our functional data demonstrate that TcUSP is a constitutively silent partner of EcR, and that none of the RXR ligands can bind and activate TcUSP. These findings together with a phylogenetic analysis suggest that RXR-USPs have undergone remarkable functional shifts during evolution and give insight into receptor-ligand binding evolution and dynamics.
About this Structure
2NXX is a Protein complex structure of sequences from Tribolium castaneum. Full crystallographic information is available from OCA.
Reference
Structural and functional characterization of a novel type of ligand-independent RXR-USP receptor., Iwema T, Billas IM, Beck Y, Bonneton F, Nierengarten H, Chaumot A, Richards G, Laudet V, Moras D, EMBO J. 2007 Aug 22;26(16):3770-82. Epub 2007 Aug 2. PMID:17673910
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