2o2l
From Proteopedia
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|PDB= 2o2l |SIZE=350|CAPTION= <scene name='initialview01'>2o2l</scene>, resolution 2.53Å | |PDB= 2o2l |SIZE=350|CAPTION= <scene name='initialview01'>2o2l</scene>, resolution 2.53Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2nzg|2NZG]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o2l OCA], [http://www.ebi.ac.uk/pdbsum/2o2l PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o2l RCSB]</span> | ||
}} | }} | ||
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[[Category: heat-labile enterotoxin]] | [[Category: heat-labile enterotoxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:11:48 2008'' |
Revision as of 01:11, 31 March 2008
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| , resolution 2.53Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Related: | 2NZG
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of human heat-labile enterotoxin in complex with a blood group A antigen analog
Overview
In a number of bacterial infections, such as Helicobacter pylori, Campylobacter jejuni and Vibrio cholerae infections, a correlation between the severity of disease and blood group phenotype of infected individuals has been observed. In the present investigation, we have studied the molecular basis of this effect for enterotoxigenic Escherichia coli (ETEC) infections. ETEC are non-invasive bacteria, which act through second messenger pathways to cause diarrhea. It has been suggested that the major virulence factor of ETEC from human isolates, i.e. the human heat-labile enterotoxin (hLT), recognizes certain blood group epitopes, although the molecular basis of blood group antigen recognition is unknown. The 2.5 A crystal structure of the receptor-binding B-subunit of hLT in complex with the blood group A antigen analog GalNAcalpha3(Fucalpha2)Galbeta4(Fucalpha3)Glcbeta provides evidence of a previously unknown binding site in the native toxin. The structure reveals the molecular interactions underlying blood group antigen recognition and suggests how this protein can discriminate between different blood group epitopes. These results support the previously debated role of hLT in the blood group dependence of ETEC infections. Similar observations regarding the closely related cholera toxin in V. cholera infections are also discussed.
About this Structure
2O2L is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Blood group antigen recognition by Escherichia coli heat-labile enterotoxin., Holmner A, Askarieh G, Okvist M, Krengel U, J Mol Biol. 2007 Aug 17;371(3):754-64. Epub 2007 May 31. PMID:17586525
Page seeded by OCA on Mon Mar 31 04:11:48 2008
