2o3d
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= SFRS1, ASF, SF2, SF2P33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= SFRS1, ASF, SF2, SF2P33 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o3d OCA], [http://www.ebi.ac.uk/pdbsum/2o3d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o3d RCSB]</span> | ||
}} | }} | ||
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[[Category: rrm domain]] | [[Category: rrm domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:12:08 2008'' |
Revision as of 01:12, 31 March 2008
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| Gene: | SFRS1, ASF, SF2, SF2P33 (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of human SF2/ASF RNA recognition motif 2 (RRM2)
Overview
The serine/arginine-rich (SR) protein splicing factor 2/alternative splicing factor (SF2/ASF) has a role in splicing, stability, export and translation of messenger RNA. Here, we present the structure of the RNA recognition motif (RRM) 2 from SF2/ASF, which has an RRM fold with a considerably extended loop 5 region, containing a two-stranded beta-sheet. The loop 5 extension places the previously identified SR protein kinase 1 docking sequence largely within the RRM fold. We show that RRM2 binds to RNA in a new way, by using a tryptophan within a conserved SWQLKD motif that resides on helix alpha1, together with amino acids from strand beta2 and a histidine on loop 5. The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2.
About this Structure
2O3D is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural and functional analysis of RNA and TAP binding to SF2/ASF., Tintaru AM, Hautbergue GM, Hounslow AM, Hung ML, Lian LY, Craven CJ, Wilson SA, EMBO Rep. 2007 Aug;8(8):756-62. Epub 2007 Jul 13. PMID:17668007
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