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5wjc

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Revision as of 09:33, 18 July 2018

Crystal structure of Schizosaccharomyces pombe Mis16 in complex with Eic1

Structural highlights

5wjc is a 2 chain structure with sequence from Fission yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	mis16, hat2, SPCC1672.10 (Fission yeast), SPBC27B12.02, SPBC30B4.10 (Fission yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[HAT2_SCHPO] Regulatory subunit of the histone acetylase B (HAT-B) complex (By similarity). The complex acetylates 'Lys-12' of histone H4 which is required for telomeric silencing (By similarity). Component of the CENP-A recruiting complex that ensures the integrity of mitotic spindles through maintenance of kinetochore factors mis6/CENP-I and cnp1/CENP-A (PubMed:15369671, PubMed:24774534, PubMed:24789708, PubMed:25375240). Maintains the deacetylated state of histones specifically in the central core of the centromeres (PubMed:15369671).[UniProtKB:P39984]^[1] ^[2] ^[3] ^[4] [MIS19_SCHPO] Component of the CENP-A recruiting complex that ensures the integrity of mitotic spindles through maintenance of kinetochore factors mis6/CENP-I and cnp1/CENP-A (PubMed:24774534, PubMed:24789708, PubMed:25375240). Links mis16 and mis18 to recruit CENP-A through interacting with non-sense-mediated mRNA decay (NMD) factors and the SWI/SNF complex (PubMed:24774534). Links also mis18 with the CCAN/mis6/ctf19 complex to promote CENP-A assembly (PubMed:24789708).^[5] ^[6] ^[7]

Publication Abstract from PubMed

The Mis18 complex, composed of Mis16, Eic1, and Mis18 in fission yeast, selectively deposits the centromere-specific histone H3 variant, CENP-A(Cnp1), at centromeres. How the intact Mis18 holo-complex oligomerizes and how Mis16, a well-known ubiquitous histone H4 chaperone, plays a centromere-specific role in the Mis18 holo-complex, remain unclear. Here, we report the stoichiometry of the intact Mis18 holo-complex as (Mis16)2:(Eic1)2:(Mis18)4 using analytical ultracentrifugation. We further determine the crystal structure of Schizosaccharomyces pombe Mis16 in complex with the C-terminal portion of Eic1 (Eic1-CT). Notably, Mis16 accommodates Eic1-CT through the binding pocket normally occupied by histone H4, indicating that Eic1 and H4 compete for the same binding site, providing a mechanism for Mis16 to switch its binding partner from histone H4 to Eic1. Thus, our analyses not only determine the stoichiometry of the intact Mis18 holo-complex but also uncover the molecular mechanism by which Mis16 plays a centromere-specific role through Eic1 association.

Mis16 Switches Function from a Histone H4 Chaperone to a CENP-A(Cnp1)-Specific Assembly Factor through Eic1 Interaction.,An S, Koldewey P, Chik J, Subramanian L, Cho US Structure. 2018 May 1. pii: S0969-2126(18)30137-0. doi:, 10.1016/j.str.2018.04.012. PMID:29804820^[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hayashi T, Fujita Y, Iwasaki O, Adachi Y, Takahashi K, Yanagida M. Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Cell. 2004 Sep 17;118(6):715-29. PMID:15369671 doi:http://dx.doi.org/10.1016/j.cell.2004.09.002
↑ Hayashi T, Ebe M, Nagao K, Kokubu A, Sajiki K, Yanagida M. Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex. Genes Cells. 2014 Jul;19(7):541-54. doi: 10.1111/gtc.12152. Epub 2014 Apr 29. PMID:24774534 doi:http://dx.doi.org/10.1111/gtc.12152
↑ Subramanian L, Toda NR, Rappsilber J, Allshire RC. Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly. Open Biol. 2014 Apr 30;4:140043. doi: 10.1098/rsob.140043. PMID:24789708 doi:http://dx.doi.org/10.1098/rsob.140043
↑ Hirai H, Arai K, Kariyazono R, Yamamoto M, Sato M. The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A. PLoS One. 2014 Nov 6;9(11):e111905. doi: 10.1371/journal.pone.0111905., eCollection 2014. PMID:25375240 doi:http://dx.doi.org/10.1371/journal.pone.0111905
↑ Hayashi T, Ebe M, Nagao K, Kokubu A, Sajiki K, Yanagida M. Schizosaccharomyces pombe centromere protein Mis19 links Mis16 and Mis18 to recruit CENP-A through interacting with NMD factors and the SWI/SNF complex. Genes Cells. 2014 Jul;19(7):541-54. doi: 10.1111/gtc.12152. Epub 2014 Apr 29. PMID:24774534 doi:http://dx.doi.org/10.1111/gtc.12152
↑ Subramanian L, Toda NR, Rappsilber J, Allshire RC. Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly. Open Biol. 2014 Apr 30;4:140043. doi: 10.1098/rsob.140043. PMID:24789708 doi:http://dx.doi.org/10.1098/rsob.140043
↑ Hirai H, Arai K, Kariyazono R, Yamamoto M, Sato M. The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A. PLoS One. 2014 Nov 6;9(11):e111905. doi: 10.1371/journal.pone.0111905., eCollection 2014. PMID:25375240 doi:http://dx.doi.org/10.1371/journal.pone.0111905
↑ An S, Koldewey P, Chik J, Subramanian L, Cho US. Mis16 Switches Function from a Histone H4 Chaperone to a CENP-A(Cnp1)-Specific Assembly Factor through Eic1 Interaction. Structure. 2018 May 1. pii: S0969-2126(18)30137-0. doi:, 10.1016/j.str.2018.04.012. PMID:29804820 doi:http://dx.doi.org/10.1016/j.str.2018.04.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5wjc"

@@ Line 3: / Line 3: @@
 <StructureSection load='5wjc' size='340' side='right' caption='[[5wjc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5wjc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5wjc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WJC FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjc OCA], [http://pdbe.org/5wjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjc RCSB], [http://www.ebi.ac.uk/pdbsum/5wjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjc ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mis16, hat2, SPCC1672.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), SPBC27B12.02, SPBC30B4.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wjc OCA], [http://pdbe.org/5wjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wjc RCSB], [http://www.ebi.ac.uk/pdbsum/5wjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wjc ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Fission yeast]]
 [[Category: An, S]]
 [[Category: Chik, J]]

5wjc

From Proteopedia

Revision as of 09:33, 18 July 2018

Crystal structure of Schizosaccharomyces pombe Mis16 in complex with Eic1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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