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5xyv

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Revision as of 09:35, 18 July 2018

Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain

Structural highlights

5xyv is a 4 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	rhi (DROME), del, CG9252 (DROME)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DEL_DROME] Developmental protein involved in oogenesis (PubMed:16616913). Required for germline maintenance, stability of mitotic spindles, localization of patterning determinants, oocyte growth and fusome biogenesis in males and females (PubMed:16616913). Also required for dorso-ventral and antero-posterior patterning of oocyte and eggshell (PubMed:16616913). May be involved in microtubule function during oogenesis (PubMed:16616913). Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers (PubMed:28847004). Component of the RDC complex (rhi, del and cuff) which binds to repressive H3K9me3 marks in the piRNA clusters (PubMed:28847004). RDC promotes the bidirectional transcription of piRNA clusters at these sites by interacting with Moonshiner which forms a complex with the transcription initiation factors TfIIA-S and Trf2 (PubMed:28847004). This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark (PubMed:28847004).^[1] ^[2]

Publication Abstract from PubMed

PIWI-interacting RNAs (piRNAs) silence transposons in germ cells to maintain genome stability and animal fertility. Rhino, a rapidly evolving heterochromatin protein 1 (HP1) family protein, binds Deadlock in a species-specific manner and so defines the piRNA-producing loci in the Drosophila genome. Here, we determine the crystal structures of Rhino-Deadlock complex in Drosophila melanogaster and simulans In both species, one Rhino binds the N-terminal helix-hairpin-helix motif of one Deadlock protein through a novel interface formed by the beta-sheet in the Rhino chromoshadow domain. Disrupting the interface leads to infertility and transposon hyperactivation in flies. Our structural and functional experiments indicate that electrostatic repulsion at the interaction interface causes cross-species incompatibility between the sibling species. By determining the molecular architecture of this piRNA-producing machinery, we discover a novel HP1-partner interacting mode that is crucial to piRNA biogenesis and transposon silencing. We thus explain the cross-species incompatibility of two sibling species at the molecular level.

Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification.,Yu B, Lin YA, Parhad SS, Jin Z, Ma J, Theurkauf WE, Zhang ZZ, Huang Y EMBO Rep. 2018 Jun 1. pii: embr.201745418. doi: 10.15252/embr.201745418. PMID:29858487^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wehr K, Swan A, Schupbach T. Deadlock, a novel protein of Drosophila, is required for germline maintenance, fusome morphogenesis and axial patterning in oogenesis and associates with centrosomes in the early embryo. Dev Biol. 2006 Jun 15;294(2):406-17. doi: 10.1016/j.ydbio.2006.03.002. Epub 2006 , Apr 17. PMID:16616913 doi:http://dx.doi.org/10.1016/j.ydbio.2006.03.002
↑ Andersen PR, Tirian L, Vunjak M, Brennecke J. A heterochromatin-dependent transcription machinery drives piRNA expression. Nature. 2017 Sep 7;549(7670):54-59. doi: 10.1038/nature23482. Epub 2017 Aug 23. PMID:28847004 doi:http://dx.doi.org/10.1038/nature23482
↑ Yu B, Lin YA, Parhad SS, Jin Z, Ma J, Theurkauf WE, Zhang ZZ, Huang Y. Structural insights into Rhino-Deadlock complex for germline piRNA cluster specification. EMBO Rep. 2018 Jun 1. pii: embr.201745418. doi: 10.15252/embr.201745418. PMID:29858487 doi:http://dx.doi.org/10.15252/embr.201745418

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xyv"

@@ Line 3: / Line 3: @@
 <StructureSection load='5xyv' size='340' side='right' caption='[[5xyv]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xyv]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xyv]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XYV FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyv OCA], [http://pdbe.org/5xyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyv RCSB], [http://www.ebi.ac.uk/pdbsum/5xyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyv ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rhi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), del, CG9252 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xyv OCA], [http://pdbe.org/5xyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xyv RCSB], [http://www.ebi.ac.uk/pdbsum/5xyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xyv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Drome]]
 [[Category: Huang, Y]]
 [[Category: Yu, B W]]

5xyv

From Proteopedia

Revision as of 09:35, 18 July 2018

Crystal structure of drosophila melanogaster Rhino chromoshadow domain in complex with Deadlock N-terminal domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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