This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2jt4

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 10:12, 18 July 2018

Solution Structure of the Sla1 SH3-3-Ubiquitin Complex

Structural highlights

2jt4 is a 2 chain structure with sequence from Atcc 18824. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1ubq, 1z9z
Gene:	SLA1 (ATCC 18824), UBI1, RPL40A (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SLA1_YEAST] Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8] ^[9] ^[10] ^[11] ^[12] ^[13] ^[14] ^[15]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The SH3 domain is a protein-protein interaction module commonly found in intracellular signaling and adaptor proteins. The SH3 domains of multiple endocytic proteins have been recently implicated in binding ubiquitin, which serves as a signal for diverse cellular processes including gene regulation, endosomal sorting, and protein destruction. Here we describe the solution NMR structure of ubiquitin in complex with an SH3 domain belonging to the yeast endocytic protein Sla1. The ubiquitin binding surface of the Sla1 SH3 domain overlaps substantially with the canonical binding surface for proline-rich ligands. Like many other ubiquitin-binding motifs, the SH3 domain engages the Ile44 hydrophobic patch of ubiquitin. A phenylalanine residue located at the heart of the ubiquitin-binding surface of the SH3 domain serves as a key specificity determinant. The structure of the SH3-ubiquitin complex explains how a subset of SH3 domains has acquired this non-traditional function.

Structural basis for ubiquitin recognition by SH3 domains.,He Y, Hicke L, Radhakrishnan I J Mol Biol. 2007 Oct 12;373(1):190-6. Epub 2007 Aug 17. PMID:17765920^[16]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Holtzman DA, Yang S, Drubin DG. Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol. 1993 Aug;122(3):635-44. PMID:8335689
↑ Tang HY, Cai M. The EH-domain-containing protein Pan1 is required for normal organization of the actin cytoskeleton in Saccharomyces cerevisiae. Mol Cell Biol. 1996 Sep;16(9):4897-914. PMID:8756649
↑ Yang S, Ayscough KR, Drubin DG. A role for the actin cytoskeleton of Saccharomyces cerevisiae in bipolar bud-site selection. J Cell Biol. 1997 Jan 13;136(1):111-23. PMID:9008707
↑ Ayscough KR, Stryker J, Pokala N, Sanders M, Crews P, Drubin DG. High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A. J Cell Biol. 1997 Apr 21;137(2):399-416. PMID:9128251
↑ Ayscough KR, Eby JJ, Lila T, Dewar H, Kozminski KG, Drubin DG. Sla1p is a functionally modular component of the yeast cortical actin cytoskeleton required for correct localization of both Rho1p-GTPase and Sla2p, a protein with talin homology. Mol Biol Cell. 1999 Apr;10(4):1061-75. PMID:10198057
↑ Tang HY, Xu J, Cai M. Pan1p, End3p, and S1a1p, three yeast proteins required for normal cortical actin cytoskeleton organization, associate with each other and play essential roles in cell wall morphogenesis. Mol Cell Biol. 2000 Jan;20(1):12-25. PMID:10594004
↑ Howard JP, Hutton JL, Olson JM, Payne GS. Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis. J Cell Biol. 2002 Apr 15;157(2):315-26. Epub 2002 Apr 8. PMID:11940605 doi:http://dx.doi.org/10.1083/jcb.200110027
↑ Warren DT, Andrews PD, Gourlay CW, Ayscough KR. Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics. J Cell Sci. 2002 Apr 15;115(Pt 8):1703-15. PMID:11950888
↑ Dewar H, Warren DT, Gardiner FC, Gourlay CG, Satish N, Richardson MR, Andrews PD, Ayscough KR. Novel proteins linking the actin cytoskeleton to the endocytic machinery in Saccharomyces cerevisiae. Mol Biol Cell. 2002 Oct;13(10):3646-61. PMID:12388763 doi:http://dx.doi.org/10.1091/mbc.E02-05-0262
↑ Li H, Page N, Bussey H. Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p. Yeast. 2002 Sep 30;19(13):1097-112. PMID:12237851 doi:http://dx.doi.org/10.1002/yea.904
↑ Rodal AA, Manning AL, Goode BL, Drubin DG. Negative regulation of yeast WASp by two SH3 domain-containing proteins. Curr Biol. 2003 Jun 17;13(12):1000-8. PMID:12814545
↑ Gourlay CW, Dewar H, Warren DT, Costa R, Satish N, Ayscough KR. An interaction between Sla1p and Sla2p plays a role in regulating actin dynamics and endocytosis in budding yeast. J Cell Sci. 2003 Jun 15;116(Pt 12):2551-64. Epub 2003 May 6. PMID:12734398 doi:http://dx.doi.org/10.1242/jcs.00454
↑ Stamenova SD, Dunn R, Adler AS, Hicke L. The Rsp5 ubiquitin ligase binds to and ubiquitinates members of the yeast CIN85-endophilin complex, Sla1-Rvs167. J Biol Chem. 2004 Apr 16;279(16):16017-25. Epub 2004 Feb 3. PMID:14761940 doi:http://dx.doi.org/10.1074/jbc.M313479200
↑ Rodal AA, Kozubowski L, Goode BL, Drubin DG, Hartwig JH. Actin and septin ultrastructures at the budding yeast cell cortex. Mol Biol Cell. 2005 Jan;16(1):372-84. Epub 2004 Nov 3. PMID:15525671 doi:http://dx.doi.org/10.1091/mbc.E04-08-0734
↑ Gardiner FC, Costa R, Ayscough KR. Nucleocytoplasmic trafficking is required for functioning of the adaptor protein Sla1p in endocytosis. Traffic. 2007 Apr;8(4):347-58. Epub 2007 Feb 7. PMID:17286805 doi:http://dx.doi.org/10.1111/j.1600-0854.2007.00534.x
↑ He Y, Hicke L, Radhakrishnan I. Structural basis for ubiquitin recognition by SH3 domains. J Mol Biol. 2007 Oct 12;373(1):190-6. Epub 2007 Aug 17. PMID:17765920 doi:10.1016/j.jmb.2007.07.074

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jt4"

@@ Line 1: / Line 1: @@
 ==Solution Structure of the Sla1 SH3-3-Ubiquitin Complex==
 <StructureSection load='2jt4' size='340' side='right' caption='[[2jt4]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ubq|1ubq]], [[1z9z|1z9z]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SLA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), UBI1, RPL40A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jt4 OCA], [http://pdbe.org/2jt4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jt4 RCSB], [http://www.ebi.ac.uk/pdbsum/2jt4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jt4 OCA], [http://pdbe.org/2jt4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jt4 RCSB], [http://www.ebi.ac.uk/pdbsum/2jt4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jt4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/2jt4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/2jt4_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 39: / Line 40: @@
 [[Category: Radhakrishnan, I]]
 [[Category: Actin-binding]]
+[[Category: Cytoplasm]]
 [[Category: Cytoskeleton]]
 [[Category: Dna damage]]
@@ Line 51: / Line 53: @@
 [[Category: Ubiquitin]]
 [[Category: Ubiquitin-binding motif]]
+[[Category: Ubl conjugation]]

2jt4

From Proteopedia

Revision as of 10:12, 18 July 2018

Solution Structure of the Sla1 SH3-3-Ubiquitin Complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox