2o8k
From Proteopedia
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|PDB= 2o8k |SIZE=350|CAPTION= <scene name='initialview01'>2o8k</scene> | |PDB= 2o8k |SIZE=350|CAPTION= <scene name='initialview01'>2o8k</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= rpoN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | |GENE= rpoN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o8k OCA], [http://www.ebi.ac.uk/pdbsum/2o8k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2o8k RCSB]</span> | ||
}} | }} | ||
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[[Category: transcription factor]] | [[Category: transcription factor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:14:17 2008'' |
Revision as of 01:14, 31 March 2008
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| Ligands: | , , , | ||||||
| Gene: | rpoN (Aquifex aeolicus) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element
Overview
The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP.
About this Structure
2O8K is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
Structural basis of DNA recognition by the alternative sigma-factor, sigma54., Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE, J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658
Page seeded by OCA on Mon Mar 31 04:14:17 2008
