2obm
From Proteopedia
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|PDB= 2obm |SIZE=350|CAPTION= <scene name='initialview01'>2obm</scene>, resolution 2.25Å | |PDB= 2obm |SIZE=350|CAPTION= <scene name='initialview01'>2obm</scene>, resolution 2.25Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= escN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= escN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2obl|2OBL]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2obm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2obm OCA], [http://www.ebi.ac.uk/pdbsum/2obm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2obm RCSB]</span> | ||
}} | }} | ||
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[[Category: Vuckovic, M.]] | [[Category: Vuckovic, M.]] | ||
[[Category: Zarivach, R.]] | [[Category: Zarivach, R.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: CA]] | ||
[[Category: atpase]] | [[Category: atpase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:15:27 2008'' |
Revision as of 01:15, 31 March 2008
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| , resolution 2.25Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | escN (Escherichia coli) | ||||||
| Related: | 2OBL
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural and biochemical analysis of a prototypical ATPase from the type III secretion system of pathogenic bacteria
Overview
The type III secretion system (T3SS) ATPase is the conserved and essential inner-membrane component involved in the initial stages of selective secretion of specialized T3SS virulence effector proteins from the bacterial cytoplasm through to the infected host cell, a process crucial to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal structure of the catalytic domain of the prototypical T3SS ATPase EscN from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in vivo mutational analysis, our data show that the T3SS ATPases share similarity with the F1 ATPases but have important structural and sequence differences that dictate their unique secretory role. We also show that T3SS ATPase activity is dependent on EscN oligomerization and describe the molecular features and possible functional implications of a hexameric ring model.
About this Structure
2OBM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural analysis of a prototypical ATPase from the type III secretion system., Zarivach R, Vuckovic M, Deng W, Finlay BB, Strynadka NC, Nat Struct Mol Biol. 2007 Feb;14(2):131-7. Epub 2007 Jan 21. PMID:17237797
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