2oeh
From Proteopedia
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|PDB= 2oeh |SIZE=350|CAPTION= <scene name='initialview01'>2oeh</scene> | |PDB= 2oeh |SIZE=350|CAPTION= <scene name='initialview01'>2oeh</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= ARID5B, DESRT, MRF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= ARID5B, DESRT, MRF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oeh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oeh OCA], [http://www.ebi.ac.uk/pdbsum/2oeh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oeh RCSB]</span> | ||
}} | }} | ||
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[[Category: transcription/dna complex]] | [[Category: transcription/dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:16:39 2008'' |
Revision as of 01:16, 31 March 2008
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| Ligands: | , , , | ||||||
| Gene: | ARID5B, DESRT, MRF2 (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Determination of the Three-dimensional Structure of the Mrf2-DNA Complex Using Paramagnetic Spin Labeling
Overview
Understanding the mechanism of protein-DNA interactions at the molecular level is one of the main focuses in structural and molecular biological investigations. At present, NMR spectroscopy is the only approach that can provide atomic details of protein-DNA recognition in solution. However, determining the structures of protein-DNA complexes using NMR spectroscopy has been dependent on the observation of intermolecular nuclear Overhauser effects (NOE) and their assignments, which are difficult to obtain in many cases. In this study, we have shown that intermolecular distance constraints derived from a single spin-label in combination with docking calculations have defined many specific contacts of the complex between the AT-rich interaction domain (ARID) of Mrf2 and its target DNA. Mrf2 contacts DNA mainly using the two flexible loops, L1 and L2. While the L1 loop contacts the phosphate backbone, L2 and several residues in the adjacent helices interact with AT base pairs in the major groove of DNA. Despite the structural diversity in the ARID family of DNA-binding proteins, Mrf2 maintains contacts with DNA similar to those observed in the homologous Dri-DNA complex.
About this Structure
2OEH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Determination of the three-dimensional structure of the Mrf2-DNA complex using paramagnetic spin labeling., Cai S, Zhu L, Zhang Z, Chen Y, Biochemistry. 2007 May 1;46(17):4943-50. Epub 2007 Apr 4. PMID:17407261
Page seeded by OCA on Mon Mar 31 04:16:39 2008
Categories: Homo sapiens | Single protein | Cai, S. | Zhang, Z. | Zhu, L. | Complex | Dna | Mrf2 | Nmr | Spin labeling | Transcription/dna complex
