2ofq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 7: Line 7:
|ACTIVITY=
|ACTIVITY=
|GENE= traO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=179945 IncN plasmid R46]), traN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=179945 IncN plasmid R46])
|GENE= traO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=179945 IncN plasmid R46]), traN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=179945 IncN plasmid R46])
 +
|DOMAIN=
 +
|RELATEDENTRY=
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ofq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofq OCA], [http://www.ebi.ac.uk/pdbsum/2ofq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ofq RCSB]</span>
}}
}}
Line 30: Line 33:
[[Category: trao]]
[[Category: trao]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:59:05 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:17:09 2008''

Revision as of 01:17, 31 March 2008

Image:2ofq.gif


PDB ID 2ofq

Drag the structure with the mouse to rotate
Gene: traO (IncN plasmid R46), traN (IncN plasmid R46)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



NMR Solution Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system


Overview

Type IV secretion (T4S) systems translocate DNA and protein effectors through the double membrane of Gram-negative bacteria. The paradigmatic T4S system in Agrobacterium tumefaciens is assembled from 11 VirB subunits and VirD4. Two subunits, VirB9 and VirB7, form an important stabilizing complex in the outer membrane. We describe here the NMR structure of a complex between the C-terminal domain of the VirB9 homolog TraO (TraO(CT)), bound to VirB7-like TraN from plasmid pKM101. TraO(CT) forms a beta-sandwich around which TraN winds. Structure-based mutations in VirB7 and VirB9 of A. tumefaciens show that the heterodimer interface is conserved. Opposite this interface, the TraO structure shows a protruding three-stranded beta-appendage, and here, we supply evidence that the corresponding region of VirB9 of A. tumefaciens inserts in the membrane and protrudes extracellularly. This complex structure elucidates the molecular basis for the interaction between two essential components of a T4S system.

About this Structure

2OFQ is a Protein complex structure of sequences from Incn plasmid r46. Full crystallographic information is available from OCA.

Reference

NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system., Bayliss R, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll PC, Waksman G, Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1673-8. Epub 2007 Jan 23. PMID:17244707

Page seeded by OCA on Mon Mar 31 04:17:09 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ofq"
Personal tools