2oiu
From Proteopedia
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|PDB= 2oiu |SIZE=350|CAPTION= <scene name='initialview01'>2oiu</scene>, resolution 2.60Å | |PDB= 2oiu |SIZE=350|CAPTION= <scene name='initialview01'>2oiu</scene>, resolution 2.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oiu OCA], [http://www.ebi.ac.uk/pdbsum/2oiu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oiu RCSB]</span> | ||
}} | }} | ||
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[[Category: Robertson, M P]] | [[Category: Robertson, M P]] | ||
[[Category: Scott, W G.]] | [[Category: Scott, W G.]] | ||
| - | [[Category: MG]] | ||
[[Category: active site]] | [[Category: active site]] | ||
[[Category: in vitro selection]] | [[Category: in vitro selection]] | ||
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[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:18:24 2008'' |
Revision as of 01:18, 31 March 2008
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| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
L1 Ribozyme Ligase circular adduct
Overview
Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.
About this Structure
2OIU is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The structural basis of ribozyme-catalyzed RNA assembly., Robertson MP, Scott WG, Science. 2007 Mar 16;315(5818):1549-53. PMID:17363667
Page seeded by OCA on Mon Mar 31 04:18:24 2008
