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2lg5

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Revision as of 19:34, 1 August 2018

NMR structure of Chicken AvBD2 defensin

Structural highlights

2lg5 is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2lg6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GLL2_CHICK] Potent antibacterial activity against the Gram-negative bacterium E.coli ML-35, and against the Gram-positive bacterium L.monocytogenes EGD. Lacks antifungal activity against C.albicans.^[1]

Publication Abstract from PubMed

Numerous beta-defensins have been identified in birds, and the potential use of these peptides as alternatives to antibiotics has been proposed, in particular to fight antibiotic-resistant and zoonotic bacterial species. Little is known about the mechanism of antibacterial activity of avian beta-defensins, and this study was carried out to obtain initial insights into the involvement of structural features or specific residues in the antimicrobial activity of chicken AvBD2. Chicken AvBD2 and its enantiomeric counterpart were chemically synthesized. Peptide elongation and oxidative folding were both optimized. The similar antimicrobial activity measured for both L- and D-proteins clearly indicates that there is no chiral partner. Therefore, the bacterial membrane is in all likelihood the primary target. Moreover, this work indicates that the three-dimensional fold is required for an optimal antimicrobial activity, in particular for gram-positive bacterial strains. The three-dimensional NMR structure of chicken AvBD2 defensin displays the structural three-stranded antiparallel beta-sheet characteristic of beta-defensins. The surface of the molecule does not display any amphipathic character. In light of this new structure and of the king penguin AvBD103b defensin structure, the consensus sequence of the avian beta-defensin family was analyzed. Well conserved residues were highlighted, and the potential strategic role of the lysine 31 residue of AvBD2 was emphasized. The synthetic AvBD2-K31A variant displayed substantial N-terminal structural modifications and a dramatic decrease in activity. Taken together, these results demonstrate the structural as well as the functional role of the critical lysine 31 residue in antimicrobial activity.

Initial insights into structure-activity relationships of avian defensins.,Derache C, Meudal H, Aucagne V, Mark KJ, Cadene M, Delmas AF, Lalmanach AC, Landon C J Biol Chem. 2012 Mar 2;287(10):7746-55. Epub 2011 Dec 27. PMID:22205704^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Harwig SS, Swiderek KM, Kokryakov VN, Tan L, Lee TD, Panyutich EA, Aleshina GM, Shamova OV, Lehrer RI. Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes. FEBS Lett. 1994 Apr 11;342(3):281-5. PMID:8150085
↑ Derache C, Meudal H, Aucagne V, Mark KJ, Cadene M, Delmas AF, Lalmanach AC, Landon C. Initial insights into structure-activity relationships of avian defensins. J Biol Chem. 2012 Mar 2;287(10):7746-55. Epub 2011 Dec 27. PMID:22205704 doi:10.1074/jbc.M111.312108

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2lg5"

Categories: Landon, C | Meudal, H | Antimicrobial protein

@@ Line 1: / Line 1: @@
 ==NMR structure of Chicken AvBD2 defensin==
 <StructureSection load='2lg5' size='340' side='right' caption='[[2lg5]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2lg5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LG5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LG5 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lg6|2lg6]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lg5 OCA], [http://pdbe.org/2lg5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lg5 RCSB], [http://www.ebi.ac.uk/pdbsum/2lg5 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lg5 OCA], [http://pdbe.org/2lg5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lg5 RCSB], [http://www.ebi.ac.uk/pdbsum/2lg5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lg5 ProSAT]</span></td></tr>
 </table>
 == Function ==

2lg5

From Proteopedia

Revision as of 19:34, 1 August 2018

NMR structure of Chicken AvBD2 defensin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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