2oqn
From Proteopedia
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|PDB= 2oqn |SIZE=350|CAPTION= <scene name='initialview01'>2oqn</scene>, resolution 1.90Å | |PDB= 2oqn |SIZE=350|CAPTION= <scene name='initialview01'>2oqn</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=TAR:D(-)-TARTARIC ACID'>TAR</scene> | + | |LIGAND= <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqn OCA], [http://www.ebi.ac.uk/pdbsum/2oqn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oqn RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Hao, Q.]] | [[Category: Hao, Q.]] | ||
[[Category: Kim, C U.]] | [[Category: Kim, C U.]] | ||
| - | [[Category: TAR]] | ||
[[Category: capillary cryoprotection]] | [[Category: capillary cryoprotection]] | ||
[[Category: high pressure cryocooling]] | [[Category: high pressure cryocooling]] | ||
[[Category: sulfur sad phasing]] | [[Category: sulfur sad phasing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:43 2008'' |
Revision as of 01:21, 31 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths
Overview
Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.
About this Structure
2OQN is a Single protein structure of sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA.
Reference
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791
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