This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2oqn
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2oqn |SIZE=350|CAPTION= <scene name='initialview01'>2oqn</scene>, resolution 1.90Å | |PDB= 2oqn |SIZE=350|CAPTION= <scene name='initialview01'>2oqn</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=TAR:D(-)-TARTARIC ACID'>TAR</scene> | + | |LIGAND= <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2oqn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqn OCA], [http://www.ebi.ac.uk/pdbsum/2oqn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2oqn RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Hao, Q.]] | [[Category: Hao, Q.]] | ||
[[Category: Kim, C U.]] | [[Category: Kim, C U.]] | ||
| - | [[Category: TAR]] | ||
[[Category: capillary cryoprotection]] | [[Category: capillary cryoprotection]] | ||
[[Category: high pressure cryocooling]] | [[Category: high pressure cryocooling]] | ||
[[Category: sulfur sad phasing]] | [[Category: sulfur sad phasing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:21:43 2008'' |
Revision as of 01:21, 31 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths
Overview
Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.
About this Structure
2OQN is a Single protein structure of sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA.
Reference
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791
Page seeded by OCA on Mon Mar 31 04:21:43 2008
