2ost
From Proteopedia
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|PDB= 2ost |SIZE=350|CAPTION= <scene name='initialview01'>2ost</scene>, resolution 3.100Å | |PDB= 2ost |SIZE=350|CAPTION= <scene name='initialview01'>2ost</scene>, resolution 3.100Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [http://www.ebi.ac.uk/pdbsum/2ost PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB]</span> | ||
}} | }} | ||
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[[Category: Stoddard, B L.]] | [[Category: Stoddard, B L.]] | ||
[[Category: Zhao, L.]] | [[Category: Zhao, L.]] | ||
| - | [[Category: CA]] | ||
[[Category: group i intron]] | [[Category: group i intron]] | ||
[[Category: homing endonuclease]] | [[Category: homing endonuclease]] | ||
| - | [[Category: pd-(d/e)-xk motif]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | [[Category: restriction enzyme fold]] | + | [[Category: restriction enzyme fold,pd-(d/e)-xk motif]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:22:35 2008'' |
Revision as of 01:22, 31 March 2008
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| , resolution 3.100Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The structure of a bacterial homing endonuclease : I-Ssp6803I
Overview
The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
About this Structure
2OST is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.
Reference
The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif., Zhao L, Bonocora RP, Shub DA, Stoddard BL, EMBO J. 2007 May 2;26(9):2432-42. Epub 2007 Apr 5. PMID:17410205
Page seeded by OCA on Mon Mar 31 04:22:35 2008
