5ls0

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(Difference between revisions)

Revision as of 22:02, 9 August 2018

Crystal structure of Inorganic Pyrophosphatase PPA1 from Arabidopsis thaliana

Structural highlights

5ls0 is a 2 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4lug
Gene:	PPA1, At1g01050, T25K16.5 (ARATH)
Activity:	Inorganic diphosphatase, with EC number 3.6.1.1
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IPYR1_ARATH] Catalyzes the irreversible hydrolysis of pyrophosphate (PPi) to phosphate. The MgPPi(2-) complex binds to the enzyme only after a free Mg(2+) ion has bound (Ref.9). No activity with glycerol-3-phosphate, glucose-6-phosphate, p-nitrophenylphosphate, ADP, NADP(+), NAD(+),NADH, NADPH or phosphoribosyl pyrophosphate as substrates (Ref.9). Controls the equilibrium of gluconeogenic reactions in the heterotrophic growth phase of early seedling establishment. Determinates the rate of cytosolic glycolysis, providing carbon for seed storage lipid accumulation (PubMed:22566496).^[1] [REFERENCE:9]

Publication Abstract from PubMed

Inorganic pyrophosphatase (PPase) is a ubiquitous cytosolic enzyme which catalyzes the hydrolysis of inorganic pyrophosphate (PPi) to orthophosphate (Pi). The crystal structure of inorganic pyrophosphatase from Helicobacter pylori (H-PPase) has been solved by MAD and refined to an R factor of 20.6% at 2.6 A resolution. The crystallographic asymmetric unit contains a homohexameric H-PPase arranged as a dimer of trimers. While most of the structural elements of PPases are highly conserved in H-PPase, some unique structural features are localized in the flexible loops near the active site, suggesting that the structural flexibility of these loops is required for the catalytic efficiency of PPase.

Structure of inorganic pyrophosphatase from Helicobacter pylori.,Wu CA, Lokanath NK, Kim DY, Park HJ, Hwang HY, Kim ST, Suh SW, Kim KK Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1459-64. Epub 2005, Oct 19. PMID:16239722^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Meyer K, Stecca KL, Ewell-Hicks K, Allen SM, Everard JD. Oil and protein accumulation in developing seeds is influenced by the expression of a cytosolic pyrophosphatase in Arabidopsis. Plant Physiol. 2012 Jul;159(3):1221-34. doi: 10.1104/pp.112.198309. Epub 2012 May, 7. PMID:22566496 doi:http://dx.doi.org/10.1104/pp.112.198309
↑ Wu CA, Lokanath NK, Kim DY, Park HJ, Hwang HY, Kim ST, Suh SW, Kim KK. Structure of inorganic pyrophosphatase from Helicobacter pylori. Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1459-64. Epub 2005, Oct 19. PMID:16239722 doi:10.1107/S0907444905025667

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ls0"

@@ Line 3: / Line 3: @@
 <StructureSection load='5ls0' size='340' side='right' caption='[[5ls0]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ls0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LS0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ls0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LS0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LS0 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lug|4lug]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PPA1, At1g01050, T25K16.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inorganic_diphosphatase Inorganic diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.1 3.6.1.1] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ls0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ls0 OCA], [http://pdbe.org/5ls0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ls0 RCSB], [http://www.ebi.ac.uk/pdbsum/5ls0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ls0 ProSAT]</span></td></tr>
@@ Line 20: / Line 21: @@
 </div>
 <div class="pdbe-citations 5ls0" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Inorganic pyrophosphatase|Inorganic pyrophosphatase]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Arath]]
 [[Category: Inorganic diphosphatase]]
 [[Category: Grzechowiak, M]]

5ls0

From Proteopedia

Revision as of 22:02, 9 August 2018

Crystal structure of Inorganic Pyrophosphatase PPA1 from Arabidopsis thaliana

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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