6gdt

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m (Protected "6gdt" [edit=sysop:move=sysop])
Current revision (16:22, 15 August 2018) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 6gdt is ON HOLD
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==Crystal structure of exo-glucosidase/glucosaminidase VC0615 from Vibrio Cholerae==
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<StructureSection load='6gdt' size='340' side='right' caption='[[6gdt]], [[Resolution|resolution]] 3.17&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6gdt]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GDT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gdt OCA], [http://pdbe.org/6gdt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gdt RCSB], [http://www.ebi.ac.uk/pdbsum/6gdt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gdt ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glycoside hydrolase family 9 (GH9) of carbohydrate-processing enzymes primarily consists of inverting endoglucanases. A subgroup of GH9 enzymes are believed to act as exo-glucosidases or exo-glucosaminidases, with many being found in organisms of the family Vibrionaceae, where they are proposed to function within the chitin-catabolism pathway. Here, it is shown that the GH9 enzyme from the pathogen Vibrio cholerae (hereafter referred to as VC0615) is active on both chitosan-derived and beta-glucoside substrates. The structure of VC0615 at 3.17 A resolution is reported from a crystal form with poor diffraction and lattice disorder. VC0615 was highly refractory to crystallization efforts, with crystals only appearing using a high protein concentration under conditions containing the precipitant poly-gamma-glutamic acid (PGA). The structure is highly mobile within the crystal lattice, which is likely to reflect steric clashes between symmetry molecules which destabilize crystal packing. The overall tertiary structure of VC0615 is well resolved even at 3.17 A resolution, which has allowed the structural basis for the exo-glucosidase/glucosaminidase activity of this enzyme to be investigated.
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Authors: Wu, L., Davies, G.J.
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Structure of the GH9 glucosidase/glucosaminidase from Vibrio cholerae.,Wu L, Davies GJ Acta Crystallogr F Struct Biol Commun. 2018 Aug 1;74(Pt 8):512-523. doi:, 10.1107/S2053230X18011019. Epub 2018 Aug 6. PMID:30084401<ref>PMID:30084401</ref>
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Description: Crystal structure of exo-glucosidase/glucosaminidase VC0615 from Vibrio Cholerae
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 6gdt" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Davies, G J]]
[[Category: Wu, L]]
[[Category: Wu, L]]
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[[Category: Davies, G.J]]
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[[Category: Carbohydrate]]
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[[Category: Gh9]]
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[[Category: Glucosaminidase]]
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[[Category: Glucosidase]]
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[[Category: Hydrolase]]

Current revision

Crystal structure of exo-glucosidase/glucosaminidase VC0615 from Vibrio Cholerae

6gdt, resolution 3.17Å

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