2p1o
From Proteopedia
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|PDB= 2p1o |SIZE=350|CAPTION= <scene name='initialview01'>2p1o</scene>, resolution 1.90Å | |PDB= 2p1o |SIZE=350|CAPTION= <scene name='initialview01'>2p1o</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene> | + | |LIGAND= <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=NLA:NAPHTHALEN-1-YL-ACETIC+ACID'>NLA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= SKP1A, ASK1, SKP1, UIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), TIR1, FBL1, WEI1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | |GENE= SKP1A, ASK1, SKP1, UIP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]), TIR1, FBL1, WEI1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2p1m|2P1M]], [[2p1n|2P1N]], [[2p1p|2P1P]], [[2p1q|2P1Q]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p1o OCA], [http://www.ebi.ac.uk/pdbsum/2p1o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p1o RCSB]</span> | ||
}} | }} | ||
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[[Category: Tan, X.]] | [[Category: Tan, X.]] | ||
[[Category: Zheng, N.]] | [[Category: Zheng, N.]] | ||
| - | [[Category: IHP]] | ||
| - | [[Category: NLA]] | ||
[[Category: f-box]] | [[Category: f-box]] | ||
[[Category: leucine rich repeat]] | [[Category: leucine rich repeat]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:27:04 2008'' |
Revision as of 01:27, 31 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | SKP1A, ASK1, SKP1, UIP1 (Arabidopsis thaliana), TIR1, FBL1, WEI1 (Arabidopsis thaliana) | ||||||
| Related: | 2P1M, 2P1N, 2P1P, 2P1Q
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Mechanism of Auxin Perception by the TIR1 ubiquitin ligase
Overview
Auxin is a pivotal plant hormone that controls many aspects of plant growth and development. Perceived by a small family of F-box proteins including transport inhibitor response 1 (TIR1), auxin regulates gene expression by promoting SCF ubiquitin-ligase-catalysed degradation of the Aux/IAA transcription repressors, but how the TIR1 F-box protein senses and becomes activated by auxin remains unclear. Here we present the crystal structures of the Arabidopsis TIR1-ASK1 complex, free and in complexes with three different auxin compounds and an Aux/IAA substrate peptide. These structures show that the leucine-rich repeat domain of TIR1 contains an unexpected inositol hexakisphosphate co-factor and recognizes auxin and the Aux/IAA polypeptide substrate through a single surface pocket. Anchored to the base of the TIR1 pocket, auxin binds to a partially promiscuous site, which can also accommodate various auxin analogues. Docked on top of auxin, the Aux/IAA substrate peptide occupies the rest of the TIR1 pocket and completely encloses the hormone-binding site. By filling in a hydrophobic cavity at the protein interface, auxin enhances the TIR1-substrate interactions by acting as a 'molecular glue'. Our results establish the first structural model of a plant hormone receptor.
About this Structure
2P1O is a Protein complex structure of sequences from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Mechanism of auxin perception by the TIR1 ubiquitin ligase., Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, Zheng N, Nature. 2007 Apr 5;446(7136):640-5. PMID:17410169
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