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2n52

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Revision as of 17:22, 15 August 2018

The solution structure of the kallikrein inhibitor SPINK6

Structural highlights

2n52 is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	SPINK6, UNQ844/PRO1782 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ISK6_HUMAN] Serine protease inhibitor selective for kallikreins. Efficiently inhibits KLK4, KLK5, KLK6, KLK7, KLK12, KLK13 and KLK14. Doesn't inhibit KLK8.^[1] ^[2]

Publication Abstract from PubMed

Kallikrein-related peptidases (KLKs) are crucial for epidermal barrier function and are involved in the proteolytic regulation of the desquamation process. Elevated KLK levels were reported in atopic dermatitis. In skin, the proteolytic activity of KLKs is regulated by specific inhibitors of the serine protease inhibitor of Kazal-type (SPINK) family. SPINK6 was shown to be expressed in human stratum corneum and is able to inhibit several KLKs such as KLK4, -5, -12, -13 and -14. In order to understand the structural traits of the specific inhibition we solved the structure of SPINK6 in solution by NMR-spectroscopy and studied its interaction with KLKs. Thereby, beside the conserved binding mode, we identified an alternate binding mode which has so far not been observed for SPINK inhibitors.

The solution structure of the kallikrein-related peptidases inhibitor SPINK6.,Jung S, Fischer J, Spudy B, Kerkow T, Sonnichsen FD, Xue L, Bonvin AM, Goettig P, Magdolen V, Meyer-Hoffert U, Grotzinger J Biochem Biophys Res Commun. 2016 Feb 26;471(1):103-8. doi:, 10.1016/j.bbrc.2016.01.172. Epub 2016 Jan 30. PMID:26828269^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Meyer-Hoffert U, Wu Z, Kantyka T, Fischer J, Latendorf T, Hansmann B, Bartels J, He Y, Glaser R, Schroder JM. Isolation of SPINK6 in human skin: selective inhibitor of kallikrein-related peptidases. J Biol Chem. 2010 Oct 15;285(42):32174-81. doi: 10.1074/jbc.M109.091850. Epub, 2010 Jul 28. PMID:20667819 doi:http://dx.doi.org/10.1074/jbc.M109.091850
↑ Kantyka T, Fischer J, Wu Z, Declercq W, Reiss K, Schroder JM, Meyer-Hoffert U. Inhibition of kallikrein-related peptidases by the serine protease inhibitor of Kazal-type 6. Peptides. 2011 Jun;32(6):1187-92. doi: 10.1016/j.peptides.2011.03.009. Epub 2011 , Mar 23. PMID:21439340 doi:http://dx.doi.org/10.1016/j.peptides.2011.03.009
↑ Jung S, Fischer J, Spudy B, Kerkow T, Sonnichsen FD, Xue L, Bonvin AM, Goettig P, Magdolen V, Meyer-Hoffert U, Grotzinger J. The solution structure of the kallikrein-related peptidases inhibitor SPINK6. Biochem Biophys Res Commun. 2016 Feb 26;471(1):103-8. doi:, 10.1016/j.bbrc.2016.01.172. Epub 2016 Jan 30. PMID:26828269 doi:http://dx.doi.org/10.1016/j.bbrc.2016.01.172

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2n52"

Categories: Human | Grotzinger, J | Hydrolase inhibitor | Inhibitor

@@ Line 3: / Line 3: @@
 <StructureSection load='2n52' size='340' side='right' caption='[[2n52]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2n52]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N52 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2n52]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2N52 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n52 OCA], [http://pdbe.org/2n52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n52 RCSB], [http://www.ebi.ac.uk/pdbsum/2n52 PDBsum]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPINK6, UNQ844/PRO1782 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2n52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n52 OCA], [http://pdbe.org/2n52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n52 RCSB], [http://www.ebi.ac.uk/pdbsum/2n52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n52 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Human]]
 [[Category: Grotzinger, J]]
 [[Category: Hydrolase inhibitor]]
 [[Category: Inhibitor]]

2n52

From Proteopedia

Revision as of 17:22, 15 August 2018

The solution structure of the kallikrein inhibitor SPINK6

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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