Carboxypeptidase

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	**[[3mtw]] – RCP + arginine derivative – ''Caulobacter vibrioides''		**[[3mtw]] – RCP + arginine derivative – ''Caulobacter vibrioides''
-	*'''Alanine carboxypeptidase'''	+	*'''D-alanyl-D-alanine carboxypeptidase'''
-	**[[3ozh]], [[3rju]] – ACP – ''Yersinia pestis''	+	**[[1w79]], [[1w8q]] – AACP – ''Actinomadura''<br />
-		+	**[[3pte]], [[1skf]] – StAACP – ''Streptomyces''<br />
-	*'''Alanyl-alanine carboxypeptidase'''	+	**[[4rye]] – MtAACP <br />
-		+	**[[5fsr]] – EcAACP <br />
-	See [[Penicillin-binding protein]]	+	**[[1es2]], [[1es3]], [[1es4]], [[1es5]], [[1esi]], [[1j9m]] – StAACP (mutant) <br />
		+	**[[1lbu]] – SaAACP + Zn – ''Streptomyces albus''<br />
		+	**[[2wke]], [[1w8y]], [[2vgj]], [[2vgk]], [[4ben]] - AcAACP + antibiotic<br />
		+	**[[1cef]], [[1ceg]], [[1hvb]], [[1pw1]], [[1pw8]], [[1pwc]], [[1pwd]], **[[1pwg]] – StAACP + antibiotic<br />
		+	**[[2xdm, [[2xk1]], [[2xln]], [[2y4a]], [[2y55]], [[2y59]], [[3zvt]], [[3zvw]], [[4b4x]], [[4b4z]] – AcAACP + boronic acid derivative<br />
		+	**[[1ikg]], [[1iki]] – StAACP + peptidoglycan fragment<br />
		+	**[[1mpl]] - StAACP + phosphonate inhibitor<br />
		+	**[[1scw]], [[1sde]] - StAACP + cyclic phosphate inhibitor<br />
		+	**[[1yqs]] - StAACP + sultam inhibitor<br />
		+	**[[3zcz]] – AcAACP + inhibitor<br />
		+	**[[5j8x]] – EcAACP + inhibitor<br />
	*β-lactamase/D-alanine carboxypeptidase		*β-lactamase/D-alanine carboxypeptidase
	**[[5evi]] - LACP – ''Pseudomonas syringae''<br />		**[[5evi]] - LACP – ''Pseudomonas syringae''<br />
		+	**[[3ozh]], [[3rju]] – ACP – ''Yersinia pestis''<br />
	*'''Glutamate carboxypeptidase II'''		*'''Glutamate carboxypeptidase II'''
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	*'''Muramoyl pentapeptide carboxypeptidase'''		*'''Muramoyl pentapeptide carboxypeptidase'''
-	**[[1lbu]] – CP – ''Streptomyces albus''<br />	+	**[[1lbu]] – SaCP<br />
	*'''Metallo-carboxypeptidase'''		*'''Metallo-carboxypeptidase'''

---

Revision as of 08:38, 16 August 2018

spinqualitylabelsall models Pig carboxypeptidase B complex with acetate and Zn+2 ions (grey) (PDB code 3wc6) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Contents 1 Function 2 Relevance 3 Structural highlights Function Carboxypeptidase (CP) cleaves the amino acid at the C terminal of a polypeptide chain. 2-guanidinoethylmercaptosuccinic acid (GEMSA) is a potent inhibitor of CP. Carboxypeptidase A (CPA) cleaves preferentially aromatic or branched-chain amino acids.[1] Carboxypeptidase B (CPB) cleaves preferentially positively charged amino acids.[2] Carboxypeptidase D (CPD) cleaves preferentially Arg or Lys at the C-terminal. Carboxypeptidase E (CPE) cleaves preferentially Arg or Lys at the C-terminal. Carboxypeptidase M (CPM) is membrane-bound and cleaves preferentially Arg or Lys. Carboxypeptidase N (CPN) is a plasma Zn metalloprotease which cleaves preferentially Arg or Lys at the C-terminal. Carboxypeptidase T (CPT) cleaves preferentially Arg, Lys or hydrophobic residues at the C-terminal. Carboxypeptidase Y (CPY) is a yeast protease which cleaves at the C-terminal. Carboxypeptidase M32 is a Zn containing metalloprotease. Glutamate carboxypeptidase (GCP) cleaves glutamate from the peptide N-acetyl-aspartyl-glutamate. Prolyl arboxypeptidase (PCP) cleaves preferentially C termini amino acids preceded by proline. Alanyl-alanine carboxypeptidase is called penicillin-binding protein. Procarboxypeptidases are zymogens which undergo cleavage in order to become an active carboxypeptidase. Relevance Glutamate carboxypeptidase II is a potential target for imaging and therapy of prostate carcinoma and attenuation of neurotoxicity. Structural highlights The is located at the bottom of a pocket open to the surface of the enzyme.[3]

3D Structures of Carboxypeptidase

Updated on 16-August-2018 {{#tree:id=OrganizedByTopic|openlevels=0|

• Carboxypeptidase A

• • 5cpa, 1yme, 1cps, 1ee3, 1cpx, 1m4l – bCPA – bovine
    
  • 1arl – bCPA
    
  • 1arm, 1eel, 1ell, 1elm – bCPA Zn-substituted
    
  • 1k9x – PfCPA apo – Pyrococcus furiosus
    
  • 1ka2, 1ka4 - PfCPA Zn-substituted
    
  • 5wvu, 3hoa – CPA – Thermus thermophilus
    
  • 2v77 – hCPA1 – human
    
  • 3dwc – CPA – Trypanosoma cruzi
    
  • 3g23 – CPA – Novosphingobium aromaticivorans
    
  • 4ox5 – SpCPA – Stretomyces pneumoniae
    
  • 5e3x – CPA – Fervidobacterium islandicum
    
  • 5giv – CPA – Deinococcus radiodurans
    

• CPA complex with small molecules

• • 3cpa – bCPA + glycyl-tyrosine
    
  • 1f57 – bCPA + cysteine
    
  • 1hdq, 1hdu, 1hee – bCPA + phenylalanine derivative
    
  • 6cpa, 7cpa, 8cpa – bCPA + phosphate derivative
    
  • 2scp, 1cbx, 1bav, 1iy7, 2rfh, 3fx6, 3fvl, 3hlp, 3huv, 3kgq – bCPA + inhibitor
    
  • 3i1u – bCPA + thiirane
    
  • 2ctb, 2ctc – bCPA + phenyl lactate
    
  • 2pcu – hCPA4 + peptide
    
  • 4uee, 4uef, 4uez – hCPA1 + peptide inhibitor
    
  • 4uf4, 5om9 – hCPA1 + inhibitor
    
  • 4oxd – SpCP + peptidoglycan
    

• CPA complex with protein

• • 1zlh – bCPA + tick inhibitor
    
  • 4cpa – bCPA + potato inhibitor
    
  • 2abz - bCPA + metallo-CP inhibitor
    
  • 3fju – hCPA1 + ascaris inhibitor
    
  • 1dtd – hCPA2 + metallo-CP inhibitor
    
  • 2bo9 – hCPA4 hydrolase domain + latexin
    
  • 4bd9 - hCPA4 hydrolase domain + SMCI inhibitor
    
  • 4a94 – hCPA4 + nerita versicolor inhibitor
    

• Procarboxypeptidase A

• • 1fon – bPCPA subunit III
    
  • 1pyt – bPCPA + proproteinase E + chymotrypsinogen C
    
  • 1jqg – PCPA – Cotton bollworm
    
  • 1aye – hPCPA2
    
  • 1o6x – hPCPA2 activation domain – NMR
    
  • 2boa – hPCPA4
    

• Carboxypeptidase B

• • 1cpb, 3dgv – bCPB
    
  • 3d4u, 3osl – bCPB + CP inhibitor
    
  • 2c1c – CPB – Corn earworm
    
  • 3glj – pCPB residues 16-416 - pig
    
  • 5jc6 – pCPB catalytic domain residues 111-416
    
  • 3wab, 3wc5, 3wc6, 3wc7, 5lyf, 5lyi, 5lyl, 4z65, 4uia, 2piy, 2piz, 2pj0, 2pj1, 2pj2, 2pj3, 2pj4, 2pj5, 2pj6, 2pj7, 2pj8, 2pj9, 2pja, 2pjb, 2pjc – pCPB catalytic domain + inhibitor
    
  • 5lyd, 4uib – pCPB catalytic domain (mutant) + inhibitor
    
  • 5lrg, 5lrj, 5lrk – pCPB catalytic domain + anabaenopepsin peptide
    
  • 3d66 – hCPB 2
    
  • 3d68 – hCPB 2 (mutant)
    
  • 3lms – hCPB 2 + protein inhibitor
    
  • 5hvf, 5hvg, 5hvh – hCPB 2 (mutant) + nanobody inhibitor
    
  • 4p10 – hCPB 2 (mutant) + inhibitor
    
  • 3d67 – hCPB 2 + GEMSA
    
  • 1zli - hCPB + tick inhibitor
    

• Procarboxypeptidase B

• • 1pba – pPCPB activation domain – NMR
    
  • 1nsa – pPCPB
    
  • 1z5r - pPCPB catalytic domain
    
  • 1kwm - hPCPB
    
  • 1pca – pPCPB + valine
    
  • 1zg7, 1zg8, 1zg9 – pPCPB + inhibitor
    

• Carboxypeptidase D

• • 1qmu – dCPD domain II - duck
    
  • 1h8l - dCPD domain II + GEMSA
    
  • 3mn8 – DmCPD + GEMSA – Drosophila melanogaster
    
  • 5aq0 – hCPD transthyretin-like domain
    

• Dipeptidyl carboxypeptidase I

• • 2iul, 2iux – hCPI (mutant)
    
  • 2c6f – hCPI N domain
    
  • 2x8y – DmCPI
    
  • 5l43 – AhCPI – Astrosporangium hypotensionis
    

• Dipeptidyl carboxypeptidase I complexes

• • 2x8z, 2x90, 2x91, 2x92, 2x93, 2x94, 2x95, 2x96, 2x97 – DmCPI + anti-hypersensitive drug
    
  • 2xhm - DmCPI + phosphate derivative
    
  • 2oc2, 2xy9, 2xyd – hCPI + phosphate derivative
    
  • 3nxq - hCPI (mutant) + phosphate derivative
    
  • 3bkk, 3bkl, 3l3n – hCPI + inhibitor
    
  • 2ydm, 3zqz – hCPI + captopril derivative
    
  • 5l44 – AhCPI + peptide inhibitor
    

• Carboxypeptidase L

• • 1ivy– hCPL

• Carboxypeptidase M

• • 1uwy – hCPM

• Carboxypeptidase N

• • 2nsm – hCPN catalytic domain

• Carboxypeptidase O

• • 5mrv – hCPO + metallo-CP inhibitor
    

• Carboxypeptidase T

• • 1obr, 3qnv – TvCPT – Thermoaclinomyces vulgaris
    
  • 3prt, 3v38, 4ihm – TvCPT (mutant)
    
  • 4djl – TvCPT + sulfamoyl phenylalanine
    
  • 4iav – TvCPT (mutant) + sulfamoyl phenylalanine
    
  • 5myh – TvCPT + sulfamoyl glutamate
    
  • 4duk – TvCPT + benzyl succinic acid
    
  • 4f8z – TvCPT + leucine derivative
    
  • 4ik2 – TvCPT (mutant) + leucine derivative
    
  • 4gm5 – TvCPT + arginine derivative
    
  • 3v7z – TvCPT + GEMSA
    

• Carboxypeptidase Y

• • 1obr, 1wpx – yCPY + CPY inhibitor - yeast

• Serine carboxypeptidase

• • 3sc2 – wSCP II – wheat
    
  • 1ysc, 1cpy – ySCP
    
  • 2j7v – SeSCP – Synechococcus elongatus
    
  • 2j9o – SeSCP (mutant)

• Serine carboxypeptidase complexes

• • 1whs, 1wht - wSCP II + benzylsuccinate
    
  • 1bcr, 1bcs - wSCP II + antipain + arginine
    
  • 2j8y – SeSCP + penicillin
    
  • 2jbf - SeSCP (mutant) + penicillin

• Arginine carboxypeptidase

• • 3be7, 3dug – RCP
    
  • 3mtw – RCP + arginine derivative – Caulobacter vibrioides

• D-alanyl-D-alanine carboxypeptidase

• • 1w79, 1w8q – AACP – Actinomadura
    
  • 3pte, 1skf – StAACP – Streptomyces
    
  • 4rye – MtAACP
    
  • 5fsr – EcAACP
    
  • 1es2, 1es3, 1es4, 1es5, 1esi, 1j9m – StAACP (mutant)
    
  • 1lbu – SaAACP + Zn – Streptomyces albus
    
  • 2wke, 1w8y, 2vgj, 2vgk, 4ben - AcAACP + antibiotic
    
  • 1cef, 1ceg, 1hvb, 1pw1, 1pw8, 1pwc, 1pwd, **1pwg – StAACP + antibiotic
    
  • [[2xdm, 2xk1, 2xln, 2y4a, 2y55, 2y59, 3zvt, 3zvw, 4b4x, 4b4z – AcAACP + boronic acid derivative
    
  • 1ikg, 1iki – StAACP + peptidoglycan fragment
    
  • 1mpl - StAACP + phosphonate inhibitor
    
  • 1scw, 1sde - StAACP + cyclic phosphate inhibitor
    
  • 1yqs - StAACP + sultam inhibitor
    
  • 3zcz – AcAACP + inhibitor
    
  • 5j8x – EcAACP + inhibitor
    

• β-lactamase/D-alanine carboxypeptidase

• • 5evi - LACP – Pseudomonas syringae
    
  • 3ozh, 3rju – ACP – Yersinia pestis
    

• Glutamate carboxypeptidase II

• • 1z8l, 2oot – hECP II
    
  • 2c6c, 2jbj, 2jbk, 2or4, 3d7d, 3d7f, 3d7g, 3d7h, 3iww, 3sjf, 4jyw, 4jz0, 4lqg, 5ely, 5d29, 4x3r, 4w9y – hECP II + inhibitor
    
  • 4p44, 4p45, 4p4e, 4p4d, 4p4f, 4p4i, 4p4j, 4p4b – hECP II + phosphoramidate inhibitor
    
  • 4ngm, 4ngn, 4ngp, 4ngq, 4ngr, 4ngs, 4ngt, 4oc0, 4oc1, 4oc2, 4oc3, 4oc4, 4oc5, 4ome - hECP II + urea derivative
    
  • 2c6g - hECP II + glutamate
    
  • 4mcr, 4mcs - hECP II (mutant) + glutamate
    
  • 4mcp, 4mcq, 5f09 - hECP II (mutant) + glutamate derivative
    
  • 3bhx, 3bi0, 3bi1 - hECP II + transition state analog
    
  • 2cij – hECP II + methionine
    
  • 2pvv - hECP II + serine-sulfate
    
  • 2c6p, 2pvw - hECP II + phosphate derivative
    
  • 3rbu – hECP II + PMPA
    
  • 3bxm – hECP II (mutant) + dipeptide
    
  • 3sje, 3sjg, 3sjx - hECP II (mutant) + inhibitor
    
  • 2xef, 2xeg, 2xei, 2xej – hECP II + antibody-recruiting molecule
    
  • 1cg2 – ECP II – Pseudomonas

• Glutamate carboxypeptidase III

• • 3fec – hECP III
    
  • 3fed - hECP III + transition state analog
    
  • 3fee – hECP III + inhibitor
    
  • 3ff3 - hECP III + glutamate

• Prolyl carboxypeptidase

• • 3n2z - hPCP

• Angiotensin-converting enzyme-related carboxypeptidase

See Angiotensin-Converting Enzyme

• M32 carboxypeptidase

• • 3hq2 – BsCP

• Muramoyl pentapeptide carboxypeptidase

• • 1lbu – SaCP
    

• Metallo-carboxypeptidase

• • 4a37 – PaCP – Pseudomonas aeruginosa
    
  • 4a38 – PaCP + benzyl succinic acid
    
  • 4a39 – PaCP + GEMSA
    

}}

References

1. ↑ Fernandez D, Boix E, Pallares I, Aviles FX, Vendrell J. Structural and Functional Analysis of the Complex between Citrate and the Zinc Peptidase Carboxypeptidase A. Enzyme Res. 2011;2011:128676. doi: 10.4061/2011/128676. Epub 2011 Jul 25. PMID:21804935 doi:10.4061/2011/128676
2. ↑ Adler M, Bryant J, Buckman B, Islam I, Larsen B, Finster S, Kent L, May K, Mohan R, Yuan S, Whitlow M. Crystal structures of potent thiol-based inhibitors bound to carboxypeptidase B. Biochemistry. 2005 Jul 5;44(26):9339-47. PMID:15982000 doi:http://dx.doi.org/10.1021/bi0501941
3. ↑ Yoshimoto N, Itoh T, Inaba Y, Ishii H, Yamamoto K. Structural Basis for Inhibition of Carboxypeptidase B by Selenium-Containing Inhibitor: Selenium Coordinates to Zinc in Enzyme. J Med Chem. 2013 Sep 24. PMID:24010887 doi:10.1021/jm400816v