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| - | | + | #REDIRECT [[5zge]] This PDB entry is obsolete and replaced by 5zge |
| - | ==Crystal structure of NDM-1 in complex with hydrolyzed ampicillin==
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| - | <StructureSection load='3q6x' size='340' side='right' caption='[[3q6x]], [[Resolution|resolution]] 1.30Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[3q6x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. The July 2015 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''New Delhi Metallo-b-Lactamase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2015_7 10.2210/rcsb_pdb/mom_2015_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q6X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q6X FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=ZZ7:(2R,4S)-2-[(R)-{[(2R)-2-AMINO-2-PHENYLACETYL]AMINO}(CARBOXY)METHYL]-5,5-DIMETHYL-1,3-THIAZOLIDINE-4-CARBOXYLIC+ACID'>ZZ7</scene></td></tr>
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| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">blaNDM-1, NDM-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q6x OCA], [http://pdbe.org/3q6x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q6x RCSB], [http://www.ebi.ac.uk/pdbsum/3q6x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q6x ProSAT]</span></td></tr>
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| - | </table>
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| - | == Function ==
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| - | [[http://www.uniprot.org/uniprot/BLAN1_KLEPN BLAN1_KLEPN]] Confers resistance to many beta-lactam antibiotics, including some carbapenems. Does not confer resistance to the polymixin colistin or the fluoroquinolone ciprofloxacin.
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Metallo-beta-lactamases (MBLs) hydrolyze most beta-lactam antibiotics, and bacteria containing this kind of enzyme pose a serious threat to the public health. The newly identified New Delhi MBL (NDM-1) is a new member of this family that shows tight binding to penicillin and cephalosporins. The rapid dissemination of NDM-1 in clinically relevant bacteria has become a global concern. However, no clinically useful inhibitors against MBLs exist, partly due to the lack of knowledge about the catalysis mechanism of this kind of enzyme. Here we report the crystal structure of this novel enzyme in complex with a hydrolyzed ampicillin at its active site at 1.3-A resolution. Structural comparison with other MBLs revealed a new hydrolysis mechanism applicable to all three subclasses of MBLs, which might help the design of mechanism based inhibitors.-Zhang, H.-M., Hao, Q. Crystal structure of NDM-1 reveals a common beta-lactam hydrolysis mechanism.
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| - | Crystal structure of NDM-1 reveals a common {beta}-lactam hydrolysis mechanism.,Zhang H, Hao Q FASEB J. 2011 Apr 20. PMID:21507902<ref>PMID:21507902</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 3q6x" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Beta-lactamase]]
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| - | [[Category: New Delhi Metallo-b-Lactamase]]
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| - | [[Category: RCSB PDB Molecule of the Month]]
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| - | [[Category: Hao, Q]]
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| - | [[Category: Zhang, H]]
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| - | [[Category: Antibiotic resistant]]
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| - | [[Category: Hydrolase-antibiotic complex]]
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| - | [[Category: Ndm-1]]
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