5ysz
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==transcriptional regulator CelR-cellobiose complex== | |
| + | <StructureSection load='5ysz' size='340' side='right' caption='[[5ysz]], [[Resolution|resolution]] 1.63Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ysz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YSZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YSZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBI:CELLOBIOSE'>CBI</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ysz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ysz OCA], [http://pdbe.org/5ysz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ysz RCSB], [http://www.ebi.ac.uk/pdbsum/5ysz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ysz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CelR is a transcriptional regulator that controls the expression of cellulases catalyzing cellulose hydrolysis. However, the structural mechanism of its regulation has remained unclear. Here, we report the first structure of CelR, in this case with cellobiose bound. CelR consists of a DNA-binding domain (DBD) and a regulatory domain (RD), and homodimerizes with each monomer bound to cellobiose. A hinge region (HR) in CelR connects the DBD with the RD, and Leu59 in the HR acts as a 'leucine lever' that transduces a transcriptional activation signal. Furthermore, an alpha4 helix mediates the ligand-binding signal for transcriptional activation. Tyr84 and Gln301 can potentially alter the ligand specificity of CelR. This study provides a pivotal step toward understanding transcription of the cellulases. | ||
| - | + | Structural and functional analyses of the cellulase transcription regulator CelR.,Fu Y, Yeom SJ, Kwon KK, Hwang J, Kim H, Woo EJ, Lee DH, Lee SG FEBS Lett. 2018 Jul 31. doi: 10.1002/1873-3468.13206. PMID:30062758<ref>PMID:30062758</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5ysz" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Fu, Y]] | [[Category: Fu, Y]] | ||
| - | [[Category: Lee, S | + | [[Category: Lee, D H]] |
| + | [[Category: Lee, S G]] | ||
| + | [[Category: Yeom, S Y]] | ||
| + | [[Category: Cellobiose binding protein]] | ||
| + | [[Category: Cellulase gene regulator]] | ||
| + | [[Category: Complex]] | ||
| + | [[Category: Laci/galr family]] | ||
| + | [[Category: Transcription]] | ||
| + | [[Category: Transcriptional regulator]] | ||
Revision as of 06:00, 22 August 2018
transcriptional regulator CelR-cellobiose complex
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