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2p8q
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= KPNB1, NTF97 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= KPNB1, NTF97 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1qgk|1QGK]], [[1ukl|1UKL]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p8q OCA], [http://www.ebi.ac.uk/pdbsum/2p8q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p8q RCSB]</span> | ||
}} | }} | ||
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[[Category: snurportin]] | [[Category: snurportin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:32:24 2008'' |
Revision as of 01:32, 31 March 2008
| |||||||
| , resolution 2.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | KPNB1, NTF97 (Homo sapiens) | ||||||
| Related: | 1QGK, 1UKL
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of human Importin beta bound to the Snurportin1 IBB-domain
Overview
The nuclear import of uridine-rich ribonucleoproteins (U snRNPs) is mediated by the transport adaptor snurportin 1 (SNP1). Similar to importin alpha, SNP1 uses an N-terminal importin beta binding (sIBB) domain to recruit the receptor importin beta and gain access to the nucleus. In this paper, we demonstrate the sIBB-domain has a bipartite nature, which contains two distinct binding determinants for importin beta. The first determinant spans residues 25-65, and includes the previously identified alphaIBB-region of homology. The second binding determinant encompasses residues 1-24 and resembles region 1011-1035 of the nucleoporin 153 (Nup153). The two binding determinants synergize within the sIBB-domain to confer a low nanomolar binding affinity for importin beta, (Kd ~2nM) in an interaction that, in vitro, is displaced by RanGppNHp. We propose that in vivo the synergy of Nup153 and nuclear RanGTP promotes translocation of U snRNPs into the nucleus.
About this Structure
2P8Q is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular basis for the recognition of snurportin 1 by importin beta., Mitrousis G, Olia AS, Walker-Kopp N, Cingolani G, J Biol Chem. 2008 Jan 9;. PMID:18187419
Page seeded by OCA on Mon Mar 31 04:32:24 2008
