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2okv

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Revision as of 07:18, 22 August 2018

c-Myc DNA Unwinding Element Binding Protein

Structural highlights

2okv is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	HARS2, C20orf88 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DTD1_HUMAN] Hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine (Potential).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Local zones of easily unwound DNA are characteristic of prokaryotic and eukaryotic replication origins. The DNA-unwinding element of the human c-myc replication origin is essential for replicator activity and is a target of the DNA-unwinding element-binding protein DUE-B in vivo. We present here the 2.0A crystal structure of DUE-B and complementary biochemical characterization of its biological activity. The structure corresponds to a dimer of the N-terminal domain of the full-length protein and contains many of the structural elements of the nucleotide binding fold. A single magnesium ion resides in the putative active site cavity, which could serve to facilitate ATP hydrolytic activity of this protein. The structure also demonstrates a notable similarity to those of tRNA-editing enzymes. Consistent with this structural homology, the N-terminal core of DUE-B is shown to display both D-aminoacyl-tRNA deacylase activity and ATPase activity. We further demonstrate that the C-terminal portion of the enzyme is disordered and not essential for dimerization. However, this region is essential for DNA binding in vitro and becomes ordered in the presence of DNA.

Structure and function of the c-myc DNA-unwinding element-binding protein DUE-B.,Kemp M, Bae B, Yu JP, Ghosh M, Leffak M, Nair SK J Biol Chem. 2007 Apr 6;282(14):10441-8. Epub 2007 Jan 30. PMID:17264083^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kemp M, Bae B, Yu JP, Ghosh M, Leffak M, Nair SK. Structure and function of the c-myc DNA-unwinding element-binding protein DUE-B. J Biol Chem. 2007 Apr 6;282(14):10441-8. Epub 2007 Jan 30. PMID:17264083 doi:10.1074/jbc.M609632200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2okv"

@@ Line 1: / Line 1: @@
 ==c-Myc DNA Unwinding Element Binding Protein==
 <StructureSection load='2okv' size='340' side='right' caption='[[2okv]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HARS2, C20orf88 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2okv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2okv OCA], [http://pdbe.org/2okv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2okv RCSB], [http://www.ebi.ac.uk/pdbsum/2okv PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2okv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2okv OCA], [http://pdbe.org/2okv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2okv RCSB], [http://www.ebi.ac.uk/pdbsum/2okv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2okv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ok/2okv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ok/2okv_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2okv

From Proteopedia

Revision as of 07:18, 22 August 2018

c-Myc DNA Unwinding Element Binding Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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