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2o31

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Revision as of 07:28, 22 August 2018

Crystal structure of the second SH3 domain from ponsin

Structural highlights

2o31 is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2o2w
Gene:	SORBS1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SRBS1_HUMAN] Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions (By similarity).[UniProtKB:Q62417]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Determination of protein crystal structures is dependent on the growth of high-quality single crystals, a process that is not always successful. Optimum crystallization conditions must be systematically sought for, and microcrystalline powders are frequently obtained in failed attempts to grow the desired crystal. In materials science, structures of samples ranging from ceramics, pharmaceuticals, zeolites, etc., can nowadays be solved, almost routinely, from powdered samples, and there seems to be no fundamental reason, except the sheer size and complexity of the structures involved, why powder diffraction should not be employed to solve structures of small proteins. Indeed, recent work has shown that the high-quality powder diffraction data can be used in the study of protein crystal structures. We report the solution, model building, and refinement of a 67-residue protein domain crystal structure, with a cell volume of 64 879 A3, from powder diffraction. The second SH3 domain of ponsin, a protein of high biological significance due to its role in cellular processes, is determined and refined to resolution limits comparable to single-crystal techniques. Our results demonstrate the power and future applicability of the powder technique in structural biology.

Second SH3 domain of ponsin solved from powder diffraction.,Margiolaki I, Wright JP, Wilmanns M, Fitch AN, Pinotsis N J Am Chem Soc. 2007 Sep 26;129(38):11865-71. Epub 2007 Sep 5. PMID:17784760^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Margiolaki I, Wright JP, Wilmanns M, Fitch AN, Pinotsis N. Second SH3 domain of ponsin solved from powder diffraction. J Am Chem Soc. 2007 Sep 26;129(38):11865-71. Epub 2007 Sep 5. PMID:17784760 doi:10.1021/ja073846c

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2o31"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the second SH3 domain from ponsin==
 <StructureSection load='2o31' size='340' side='right' caption='[[2o31]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2o2w|2o2w]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SORBS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o31 OCA], [http://pdbe.org/2o31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o31 RCSB], [http://www.ebi.ac.uk/pdbsum/2o31 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2o31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o31 OCA], [http://pdbe.org/2o31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2o31 RCSB], [http://www.ebi.ac.uk/pdbsum/2o31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2o31 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o3/2o31_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o3/2o31_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2o31

From Proteopedia

Revision as of 07:28, 22 August 2018

Crystal structure of the second SH3 domain from ponsin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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