2pjr
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2pjr |SIZE=350|CAPTION= <scene name='initialview01'>2pjr</scene>, resolution 2.9Å | |PDB= 2pjr |SIZE=350|CAPTION= <scene name='initialview01'>2pjr</scene>, resolution 2.9Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pjr OCA], [http://www.ebi.ac.uk/pdbsum/2pjr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pjr RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Velankar, S S.]] | [[Category: Velankar, S S.]] | ||
[[Category: Wigley, D B.]] | [[Category: Wigley, D B.]] | ||
| - | [[Category: SO4]] | ||
[[Category: dna]] | [[Category: dna]] | ||
[[Category: helicase pcra]] | [[Category: helicase pcra]] | ||
| Line 33: | Line 35: | ||
[[Category: product complex]] | [[Category: product complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:36:52 2008'' |
Revision as of 01:36, 31 March 2008
| |||||||
| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HELICASE PRODUCT COMPLEX
Overview
We have determined two different structures of PcrA DNA helicase complexed with the same single strand tailed DNA duplex, providing snapshots of different steps on the catalytic pathway. One of the structures is of a complex with a nonhydrolyzable analog of ATP and is thus a "substrate" complex. The other structure contains a bound sulphate ion that sits in a position equivalent to that occupied by the phosphate ion produced after ATP hydrolysis, thereby mimicking a "product" complex. In both complexes, the protein is monomeric. Large and distinct conformational changes occur on binding DNA and the nucleotide cofactor. Taken together, these structures provide evidence against an "active rolling" model for helicase action but are instead consistent with an "inchworm" mechanism.
About this Structure
2PJR is a Protein complex structure of sequences from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism., Velankar SS, Soultanas P, Dillingham MS, Subramanya HS, Wigley DB, Cell. 1999 Apr 2;97(1):75-84. PMID:10199404
Page seeded by OCA on Mon Mar 31 04:36:52 2008
