5z1x
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Laccase from Cerrena sp. RSD1== | |
| + | <StructureSection load='5z1x' size='340' side='right' caption='[[5z1x]], [[Resolution|resolution]] 1.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5z1x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerrena_sp._rsd1 Cerrena sp. rsd1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z1X FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Laccase Laccase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.3.2 1.10.3.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z1x OCA], [http://pdbe.org/5z1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z1x RCSB], [http://www.ebi.ac.uk/pdbsum/5z1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z1x ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A high-efficiency laccase, DLac, was isolated from Cerrena sp. RSD1. The kinetic studies indicate that DLac is a diffusion-limited enzyme. The crystal structure of DLac was determined to atomic resolution, and its overall structure shares high homology to monomeric laccases, but displays unique substrate-binding loops from those in other laccases. The substrate-binding residues with small side chain and the short substrate-binding loop IV broaden the substrate-binding cavity and may facilitate large substrate diffusion. Unlike highly glycosylated fungal laccases, the less-glycosylated DLac contains one highly conserved glycosylation site at N432 and an unique glycosylation site at N468. The N-glycans stabilize the substrate-binding loops and the protein structure, and the first N-acetylglucosamine is crucial for the catalytic efficiency. Additionally, a fivefold increase in protein yield is achieved via the submerged culture method for industrial applications. Database: The atomic coordinates of the structure of DLac from Cerrena sp. RSD1 and structural factors have been deposited in the RCSB Protein Data Bank (PDB ID: 5Z1X). | ||
| - | + | Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp. RSD1.,Wu MH, Lee CC, Hsiao AS, Yu SM, Wang AH, Ho TD FEBS Open Bio. 2018 Jul 3;8(8):1230-1246. doi: 10.1002/2211-5463.12459., eCollection 2018 Aug. PMID:30087829<ref>PMID:30087829</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Lee, C | + | <div class="pdbe-citations 5z1x" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Cerrena sp. rsd1]] | ||
| + | [[Category: Laccase]] | ||
| + | [[Category: Ho, T H]] | ||
| + | [[Category: Lee, C C]] | ||
| + | [[Category: Wang, A H.J]] | ||
| + | [[Category: Wu, M H]] | ||
| + | [[Category: Copper-dependent enzyme]] | ||
| + | [[Category: Oxidoreductase]] | ||
Revision as of 10:01, 5 September 2018
Crystal Structure of Laccase from Cerrena sp. RSD1
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