2pns
From Proteopedia
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|PDB= 2pns |SIZE=350|CAPTION= <scene name='initialview01'>2pns</scene>, resolution 1.90Å | |PDB= 2pns |SIZE=350|CAPTION= <scene name='initialview01'>2pns</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> | + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=THJ:TETRATHIONATE'>THJ</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1o0e|1O0E]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pns FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pns OCA], [http://www.ebi.ac.uk/pdbsum/2pns PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pns RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Ghosh, R.]] | [[Category: Ghosh, R.]] | ||
[[Category: Thakurta, P Guha.]] | [[Category: Thakurta, P Guha.]] | ||
| - | [[Category: PO4]] | ||
| - | [[Category: THJ]] | ||
[[Category: ervatamin]] | [[Category: ervatamin]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| Line 35: | Line 36: | ||
[[Category: thermostable]] | [[Category: thermostable]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:38:29 2008'' |
Revision as of 01:38, 31 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1O0E
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
1.9 Angstrom resolution crystal structure of a plant cysteine protease Ervatamin-C refinement with cDNA derived amino acid sequence
Overview
We report here the cloning and characterization of the entire cDNA of a papain-like cysteine protease from a tropical flowering plant. The 1098-bp ORF of the cDNA codify a protease precursor having a signal peptide of 19 amino acids, a cathepsin-L like N-terminal proregion of 114 amino acids, a mature enzyme part of 208 amino acids and a C-terminal proregion of 24 amino acids. The derived amino acid sequence of the mature part tallies with the thermostable cysteine protease Ervatamin-C--as was aimed at. The C-terminal proregion of the protease has altogether a different sequence pattern not observed in other members of the family and it contains a negatively charged helical zone. The three-dimensional model of the precursor, based on the homology modeling and X-ray structure, shows that the extended peptide stretch region of the N-terminal propeptide, covering the interdomain cleft, contains protruding side chains of positively charged residues. This study also indicates that the negatively charged zone of C-terminal propeptide may interact with the positively charged zone of the N-terminal propeptide in a cooperative manner in the maturation process of this enzyme.
About this Structure
2PNS is a Protein complex structure of sequences from Tabernaemontana divaricata. Full crystallographic information is available from OCA.
Reference
A thermostable cysteine protease precursor from a tropical plant contains an unusual C-terminal propeptide: cDNA cloning, sequence comparison and molecular modeling studies., Ghosh R, Dattagupta JK, Biswas S, Biochem Biophys Res Commun. 2007 Nov 3;362(4):965-70. Epub 2007 Aug 28. PMID:17767923
Page seeded by OCA on Mon Mar 31 04:38:29 2008
