2px8
From Proteopedia
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|PDB= 2px8 |SIZE=350|CAPTION= <scene name='initialview01'>2px8</scene>, resolution 2.20Å | |PDB= 2px8 |SIZE=350|CAPTION= <scene name='initialview01'>2px8</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=3PO:TRIPHOSPHATE'>3PO</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MGT:7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE'>MGT</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene> |
| - | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span> | |
|GENE= NS5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11079 Murray Valley encephalitis virus]) | |GENE= NS5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11079 Murray Valley encephalitis virus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2px2|2PX2]], [[2px4|2PX4]], [[2px5|2PX5]], [[2pxa|2PXA]], [[2pxc|2PXC]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2px8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2px8 OCA], [http://www.ebi.ac.uk/pdbsum/2px8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2px8 RCSB]</span> | ||
}} | }} | ||
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[[Category: Verma, A.]] | [[Category: Verma, A.]] | ||
[[Category: Walter, T S.]] | [[Category: Walter, T S.]] | ||
| - | [[Category: 3PO]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: MGT]] | ||
| - | [[Category: SAH]] | ||
[[Category: 3po]] | [[Category: 3po]] | ||
[[Category: 7m-gtp]] | [[Category: 7m-gtp]] | ||
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[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:41:57 2008'' |
Revision as of 01:41, 31 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Gene: | NS5 (Murray Valley encephalitis virus) | ||||||
| Activity: | RNA-directed RNA polymerase, with EC number 2.7.7.48 | ||||||
| Related: | 2PX2, 2PX4, 2PX5, 2PXA, 2PXC
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH and 7M-GTP
Overview
We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.
About this Structure
2PX8 is a Single protein structure of sequence from Murray valley encephalitis virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:17622627
Page seeded by OCA on Mon Mar 31 04:41:57 2008
Categories: Murray valley encephalitis virus | RNA-directed RNA polymerase | Single protein | Alderton, D. | Assenberg, R. | Fuller, S D. | Grimes, J M. | Hurrelbrink, R J. | OPPF, Oxford Protein Production Facility. | Owens, R J. | Ren, J. | Stuart, D I. | Verma, A. | Walter, T S. | 3po | 7m-gtp | Methyltransferase | Oppf | Oxford protein production facility | Sah | Structural genomic
