2pxj
From Proteopedia
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|PDB= 2pxj |SIZE=350|CAPTION= <scene name='initialview01'>2pxj</scene>, resolution 2.00Å | |PDB= 2pxj |SIZE=350|CAPTION= <scene name='initialview01'>2pxj</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene>, <scene name='pdbligand=OGA:N-OXALYOLGLYCINE'>OGA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= JMJD2A, JHDM3A, JMJD2, KIAA0677 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= JMJD2A, JHDM3A, JMJD2, KIAA0677 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2p5b|2P5B]], [[2gp3|2GP3]], [[2gp5|2GP5]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pxj OCA], [http://www.ebi.ac.uk/pdbsum/2pxj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2pxj RCSB]</span> | ||
}} | }} | ||
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[[Category: Zang, J.]] | [[Category: Zang, J.]] | ||
[[Category: Zhang, G.]] | [[Category: Zhang, G.]] | ||
| - | [[Category: | + | [[Category: h3k36]] |
| - | [[Category: | + | [[Category: histone demethylase]] |
| - | [[Category: | + | [[Category: jmjc]] |
| - | [[Category: jmjd2a | + | [[Category: jmjd2a]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:42:05 2008'' |
Revision as of 01:42, 31 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | JMJD2A, JHDM3A, JMJD2, KIAA0677 (Homo sapiens) | ||||||
| Related: | 2P5B, 2GP3, 2GP5
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The complex structure of JMJD2A and monomethylated H3K36 peptide
Overview
The Jumonji C domain is a catalytic motif that mediates histone lysine demethylation. The Jumonji C-containing oxygenase JMJD2A specifically demethylates tri- and dimethylated lysine-9 and lysine-36 of histone 3 (H3K9/36 me3/2). Here we present structures of the JMJD2A catalytic core complexed with methylated H3K36 peptide substrates in the presence of Fe(II) and N-oxalylglycine. We found that the interaction between JMJD2A and peptides largely involves the main chains of the enzyme and the peptide. The peptide-binding specificity is primarily determined by the primary structure of the peptide, which explains the specificity of JMJD2A for methylated H3K9 and H3K36 instead of other methylated residues such as H3K27. The specificity for a particular methyl group, however, is affected by multiple factors, such as space and the electrostatic environment in the catalytic center of the enzyme. These results provide insights into the mechanisms and specificity of histone demethylation.
About this Structure
2PXJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of the recognition of a methylated histone tail by JMJD2A., Chen Z, Zang J, Kappler J, Hong X, Crawford F, Wang Q, Lan F, Jiang C, Whetstine J, Dai S, Hansen K, Shi Y, Zhang G, Proc Natl Acad Sci U S A. 2007 Jun 26;104(26):10818-23. Epub 2007 Jun 13. PMID:17567753
Page seeded by OCA on Mon Mar 31 04:42:05 2008
Categories: Homo sapiens | Single protein | Chen, Z. | Crawford, F. | Hong, X. | Kappler, J. | Zang, J. | Zhang, G. | H3k36 | Histone demethylase | Jmjc | Jmjd2a
