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2w3o

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Revision as of 21:07, 19 September 2018

Crystal structure of the human PNKP FHA domain in complex with an XRCC1-derived phosphopeptide

Structural highlights

2w3o is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:	,
Related:	1cdz, 1xnt, 2brf, 1xna, 2d8m
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[XRCC1_HUMAN] Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Short-patch repair of DNA single-strand breaks and gaps (SSB) is coordinated by XRCC1, a scaffold protein that recruits the DNA polymerase and DNA ligase required for filling and sealing the damaged strand. XRCC1 can also recruit end-processing enzymes, such as PNK (polynucleotide kinase 3'-phosphatase), Aprataxin and APLF (aprataxin/PNK-like factor), which ensure the availability of a free 3'-hydroxyl on one side of the gap, and a 5'-phosphate group on the other, for the polymerase and ligase reactions respectively. PNK binds to a phosphorylated segment of XRCC1 (between its two C-terminal BRCT domains) via its Forkhead-associated (FHA) domain. We show here, contrary to previous studies, that the FHA domain of PNK binds specifically, and with high affinity to a multiply phosphorylated motif in XRCC1 containing a pSer-pThr dipeptide, and forms a 2:1 PNK:XRCC1 complex. The high-resolution crystal structure of a PNK-FHA-XRCC1 phosphopeptide complex reveals the basis for this unusual bis-phosphopeptide recognition, which is probably a common feature of the known XRCC1-associating end-processing enzymes.

Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK.,Ali AA, Jukes RM, Pearl LH, Oliver AW Nucleic Acids Res. 2009 Jan 20. PMID:19155274^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ali AA, Jukes RM, Pearl LH, Oliver AW. Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK. Nucleic Acids Res. 2009 Jan 20. PMID:19155274 doi:gkn1086

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2w3o"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF THE HUMAN PNKP FHA DOMAIN IN COMPLEX WITH AN XRCC1-DERIVED PHOSPHOPEPTIDE==
+==Crystal structure of the human PNKP FHA domain in complex with an XRCC1-derived phosphopeptide==
 <StructureSection load='2w3o' size='340' side='right' caption='[[2w3o]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1cdz|1cdz]], [[1xnt|1xnt]], [[2brf|2brf]], [[1xna|1xna]], [[2d8m|2d8m]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w3o OCA], [http://pdbe.org/2w3o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w3o RCSB], [http://www.ebi.ac.uk/pdbsum/2w3o PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w3o OCA], [http://pdbe.org/2w3o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w3o RCSB], [http://www.ebi.ac.uk/pdbsum/2w3o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w3o ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w3/2w3o_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w3/2w3o_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 50: / Line 51: @@
 [[Category: Phosphoprotein]]
 [[Category: Pnkp]]
+[[Category: Polymorphism]]
 [[Category: Polynucleotide kinase 3' phosphatase]]
 [[Category: Transferase]]

2w3o

From Proteopedia

Revision as of 21:07, 19 September 2018

Crystal structure of the human PNKP FHA domain in complex with an XRCC1-derived phosphopeptide

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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