2qg6
From Proteopedia
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|PDB= 2qg6 |SIZE=350|CAPTION= <scene name='initialview01'>2qg6</scene>, resolution 1.500Å | |PDB= 2qg6 |SIZE=350|CAPTION= <scene name='initialview01'>2qg6</scene>, resolution 1.500Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NMN:BETA-NICOTINAMIDE+RIBOSE+MONOPHOSPHATE'>NMN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= NRK1, C9orf95 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= NRK1, C9orf95 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg6 OCA], [http://www.ebi.ac.uk/pdbsum/2qg6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qg6 RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Tong, L.]] | [[Category: Tong, L.]] | ||
[[Category: Xiang, S.]] | [[Category: Xiang, S.]] | ||
| - | [[Category: NMN]] | ||
| - | [[Category: PO4]] | ||
[[Category: adp]] | [[Category: adp]] | ||
| - | [[Category: nad+]] | + | [[Category: nad+,nucleoside monophosphate (nmp) kinase]] |
[[Category: nicotinamide mononucleotide]] | [[Category: nicotinamide mononucleotide]] | ||
[[Category: nicotinamide riboside kinase]] | [[Category: nicotinamide riboside kinase]] | ||
[[Category: nrk]] | [[Category: nrk]] | ||
| - | [[Category: nucleoside monophosphate (nmp) kinase]] | ||
[[Category: signaling protein]] | [[Category: signaling protein]] | ||
[[Category: tiazofurin]] | [[Category: tiazofurin]] | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:48:55 2008'' |
Revision as of 01:48, 31 March 2008
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| , resolution 1.500Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | NRK1, C9orf95 (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of human nicotinamide riboside kinase (NRK1) in complex with nicotinamide mononucleotide (NMN)
Overview
Nicotinamide riboside kinase (NRK) has an important role in the biosynthesis of NAD(+) as well as the activation of tiazofurin and other NR analogs for anticancer therapy. NRK belongs to the deoxynucleoside kinase and nucleoside monophosphate (NMP) kinase superfamily, although the degree of sequence conservation is very low. We report here the crystal structures of human NRK1 in a binary complex with the reaction product nicotinamide mononucleotide (NMN) at 1.5 A resolution and in a ternary complex with ADP and tiazofurin at 2.7 A resolution. The active site is located in a groove between the central parallel beta sheet core and the LID and NMP-binding domains. The hydroxyl groups on the ribose of NR are recognized by Asp56 and Arg129, and Asp36 is the general base of the enzyme. Mutation of residues in the active site can abolish the catalytic activity of the enzyme, confirming the structural observations.
About this Structure
2QG6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human nicotinamide riboside kinase., Khan JA, Xiang S, Tong L, Structure. 2007 Aug;15(8):1005-13. PMID:17698003
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