2wgz

From Proteopedia

(Difference between revisions)

Revision as of 08:57, 26 September 2018

Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)

Structural highlights

2wgz is a 2 chain structure with sequence from Bovin. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , , ,
Related:	2vs4, 2vxl, 1vzt, 2vxm, 1gx0, 1vzx, 1o7q, 2vs5, 1gx4, 2jck, 2jcj, 2vfz, 1g93, 1k4v, 1g8o, 1gwv, 1vzu, 2vs3, 1o7o, 2jcl, 2jco, 1gww, 1fg5
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The specificities of glycosyltransferases make them useful for the synthesis of biologically active oligosaccharides, but also restrict their range of products. In substrate engineering, substrate promiscuity is enhanced by attaching removable interactive groups to weak substrates. Thus, the attachment of betap-nitrophenyl converts galactose from a poor into a good substrate of alpha-1,3-galactosyltransferase. The crystallographic structure of a complex of alpha3GT containing p-nitrophenyl-beta-galactoside shows that the p-nitrophenyl binds similarly to the N-acetylglucosamine of the substrate, N-acetyllactosamine, interacting with the indole of Trp249. p-Nitrophenyl, unlike N-acetylglucosamine, makes no H-bonds but has more non-polar interactions, making it an effective monosaccharide mimetic.

Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal).,Jamaluddin H, Tumbale P, Ferns TA, Thiyagarajan N, Brew K, Acharya KR Biochem Biophys Res Commun. 2009 Aug 7;385(4):601-4. Epub 2009 May 30. PMID:19486884^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jamaluddin H, Tumbale P, Ferns TA, Thiyagarajan N, Brew K, Acharya KR. Crystal structure of alpha-1,3-galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNPbetaGal). Biochem Biophys Res Commun. 2009 Aug 7;385(4):601-4. Epub 2009 May 30. PMID:19486884 doi:10.1016/j.bbrc.2009.05.111

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wgz"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF ALPHA-1,3 GALACTOSYLTRANSFERASE (ALPHA3GT) IN A COMPLEX WITH P-NITROPHENYL-BETA-GALACTOSIDE (PNP-BETA-GAL)==
+==Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)==
 <StructureSection load='2wgz' size='340' side='right' caption='[[2wgz]], [[Resolution|resolution]] 2.12&Aring;' scene=''>
 == Structural highlights ==
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=147:1-O-[P-NITROPHENYL]-BETA-D-GALACTOPYRANOSE'>147</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vs4|2vs4]], [[2vxl|2vxl]], [[1vzt|1vzt]], [[2vxm|2vxm]], [[1gx0|1gx0]], [[1vzx|1vzx]], [[1o7q|1o7q]], [[2vs5|2vs5]], [[1gx4|1gx4]], [[2jck|2jck]], [[2jcj|2jcj]], [[2vfz|2vfz]], [[1g93|1g93]], [[1k4v|1k4v]], [[1g8o|1g8o]], [[1gwv|1gwv]], [[1vzu|1vzu]], [[2vs3|2vs3]], [[1o7o|1o7o]], [[2jcl|2jcl]], [[2jco|2jco]], [[1gww|1gww]], [[1fg5|1fg5]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgz OCA], [http://pdbe.org/2wgz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wgz RCSB], [http://www.ebi.ac.uk/pdbsum/2wgz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgz OCA], [http://pdbe.org/2wgz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wgz RCSB], [http://www.ebi.ac.uk/pdbsum/2wgz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wgz_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wgz_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2wgz

From Proteopedia

Revision as of 08:57, 26 September 2018

Crystal structure of alpha-1,3 galactosyltransferase (alpha3GT) in a complex with p-nitrophenyl-beta-galactoside (pNP-beta-Gal)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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