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2wkd

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Revision as of 09:00, 26 September 2018

Crystal structure of a double Ile-to-Met mutant of protein ORF34 from lactococcus phage p2

Structural highlights

2wkd is a 1 chain structure with sequence from Bplp2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	2wkc
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Lactococcus lactis, a Gram-positive bacterium widely used by the dairy industry, is subject to infection by a diverse population of virulent phages, predominantly by those of the 936 group, including the siphovirus phage p2. Confronted with the negative impact of phage infection on milk fermentation, the study of the biology of lactococcal provides insight from applied and fundamental perspectives. We decided to characterize the product of the orf34 gene from lactococcus phage p2, which was considered as a candidate single-stranded DNA binding protein (SSB) due to its localization downstream of a gene coding for a single-strand annealing protein. Two-dimensional gel electrophoresis showed that ORF34(p2) is expressed in large amounts during the early phases of phage infection, suggesting an important role in this process. Gel-shift assays, surface plasmon resonance and atomic force microscopy demonstrated that ORF34(p2) interacts with single-strand DNA with nanomolar affinity. We also determined the crystal structure of ORF34(p2) and showed that it bears a variation of the typical oligonucleotide/oligosaccharide binding-fold of SSBs. Finally, we found that ORF34(p2) is able to stimulate Escherichia coli RecA-mediated homologous recombination. The specific structural and biochemical properties that distinguish ORF34(p2) from other SSB proteins are discussed.

Structure and function of phage p2 ORF34(p2), a new type of single-stranded DNA binding protein.,Scaltriti E, Tegoni M, Rivetti C, Launay H, Masson JY, Magadan AH, Tremblay D, Moineau S, Ramoni R, Lichiere J, Campanacci V, Cambillau C, Ortiz-Lombardia M Mol Microbiol. 2009 Sep;73(6):1156-70. Epub 2009 Aug 25. PMID:19719513^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Scaltriti E, Tegoni M, Rivetti C, Launay H, Masson JY, Magadan AH, Tremblay D, Moineau S, Ramoni R, Lichiere J, Campanacci V, Cambillau C, Ortiz-Lombardia M. Structure and function of phage p2 ORF34(p2), a new type of single-stranded DNA binding protein. Mol Microbiol. 2009 Sep;73(6):1156-70. Epub 2009 Aug 25. PMID:19719513 doi:10.1111/j.1365-2958.2009.06844.x

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2wkd"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF A DOUBLE ILE-TO-MET MUTANT OF PROTEIN ORF34 FROM LACTOCOCCUS PHAGE P2==
+==Crystal structure of a double Ile-to-Met mutant of protein ORF34 from lactococcus phage p2==
 <StructureSection load='2wkd' size='340' side='right' caption='[[2wkd]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2wkd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_bacteriophage_p2 Lactococcus bacteriophage p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2wkd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bplp2 Bplp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKD FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wkc|2wkc]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkd OCA], [http://pdbe.org/2wkd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wkd RCSB], [http://www.ebi.ac.uk/pdbsum/2wkd PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkd OCA], [http://pdbe.org/2wkd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wkd RCSB], [http://www.ebi.ac.uk/pdbsum/2wkd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkd ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 11: / Line 12: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wkd_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wkd_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 30: / Line 31: @@
 __TOC__
 </StructureSection>
-[[Category: Lactococcus bacteriophage p2]]
+[[Category: Bplp2]]
 [[Category: Cambillau, C]]
 [[Category: Ortiz-Lombardia, M]]

2wkd

From Proteopedia

Revision as of 09:00, 26 September 2018

Crystal structure of a double Ile-to-Met mutant of protein ORF34 from lactococcus phage p2

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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