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2qij
From Proteopedia
| Line 6: | Line 6: | ||
|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10419 Hepatitis B virus subtype adyw]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1qgt|1QGT]], [[2g34|2G34]], [[2g33|2G33]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qij OCA], [http://www.ebi.ac.uk/pdbsum/2qij PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qij RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2QIJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 2QIJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_b_virus_subtype_adyw Hepatitis b virus subtype adyw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QIJ OCA]. |
==Reference== | ==Reference== | ||
Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17671358 17671358] | Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17671358 17671358] | ||
| - | [[Category: Hepatitis b virus subtype | + | [[Category: Hepatitis b virus subtype adyw]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ho, K L.]] | [[Category: Ho, K L.]] | ||
| Line 29: | Line 32: | ||
[[Category: viral capsid protein]] | [[Category: viral capsid protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:49:35 2008'' |
Revision as of 01:49, 31 March 2008
| |||||||
| , resolution 8.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | C (Hepatitis B virus subtype adyw) | ||||||
| Related: | 1QGT, 2G34, 2G33
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data.
Overview
Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells.
About this Structure
2QIJ is a Single protein structure of sequence from Hepatitis b virus subtype adyw. Full crystallographic information is available from OCA.
Reference
Crystallization and X-ray analysis of the T = 4 particle of hepatitis B capsid protein with an N-terminal extension., Tan WS, McNae IW, Ho KL, Walkinshaw MD, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt, 8):642-7. Epub 2007 Jul 21. PMID:17671358
Page seeded by OCA on Mon Mar 31 04:49:35 2008
