2qku
From Proteopedia
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|PDB= 2qku |SIZE=350|CAPTION= <scene name='initialview01'>2qku</scene>, resolution 2.200Å | |PDB= 2qku |SIZE=350|CAPTION= <scene name='initialview01'>2qku</scene>, resolution 2.200Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= inaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | |GENE= inaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2qkt|2QKT]], [[2qkv|2QKV]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qku OCA], [http://www.ebi.ac.uk/pdbsum/2qku PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qku RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Socolich, M.]] | [[Category: Socolich, M.]] | ||
[[Category: Wall, M.]] | [[Category: Wall, M.]] | ||
| - | [[Category: | + | [[Category: disulfide bond]] |
[[Category: membrane]] | [[Category: membrane]] | ||
| - | [[Category: pdz domain | + | [[Category: pdz domain]] |
[[Category: peptide binding protein]] | [[Category: peptide binding protein]] | ||
| + | [[Category: scaffolding protein]] | ||
[[Category: sensory transduction]] | [[Category: sensory transduction]] | ||
[[Category: vision]] | [[Category: vision]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:50:24 2008'' |
Revision as of 01:50, 31 March 2008
| |||||||
| , resolution 2.200Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | inaD (Drosophila melanogaster) | ||||||
| Related: | 2QKT, 2QKV
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The 5th PDZ Domain of InaD in 10mM DTT
Overview
The INAD scaffold organizes a multiprotein complex that is essential for proper visual signaling in Drosophila photoreceptor cells. Here we show that one of the INAD PDZ domains (PDZ5) exists in a redox-dependent equilibrium between two conformations--a reduced form that is similar to the structure of other PDZ domains, and an oxidized form in which the ligand-binding site is distorted through formation of a strong intramolecular disulfide bond. We demonstrate transient light-dependent formation of this disulfide bond in vivo and find that transgenic flies expressing a mutant INAD in which PDZ5 is locked in the reduced state display severe defects in termination of visual responses and visually mediated reflex behavior. These studies demonstrate a conformational switch mechanism for PDZ domain function and suggest that INAD behaves more like a dynamic machine rather than a passive scaffold, regulating signal transduction at the millisecond timescale through cycles of conformational change.
About this Structure
2QKU is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Dynamic scaffolding in a g protein-coupled signaling system., Mishra P, Socolich M, Wall MA, Graves J, Wang Z, Ranganathan R, Cell. 2007 Oct 5;131(1):80-92. PMID:17923089
Page seeded by OCA on Mon Mar 31 04:50:24 2008
