6gv1
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of E.coli Multidrug/H+ antiporter MdfA in outward open conformation with bound Fab fragment== | |
| + | <StructureSection load='6gv1' size='340' side='right' caption='[[6gv1]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6gv1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GV1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gv1 OCA], [http://pdbe.org/6gv1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gv1 RCSB], [http://www.ebi.ac.uk/pdbsum/6gv1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gv1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/MDFA_ECOLI MDFA_ECOLI]] Efflux pump driven by the proton motive force. Confers resistance to a broad spectrum of chemically unrelated drugs. Confers resistance to a diverse group of cationic or zwitterionic lipophilic compounds such as ethidium bromide, tetraphenylphosphonium, rhodamine, daunomycin, benzalkonium, rifampicin, tetracycline, puromycin, and to chemically unrelated, clinically important antibiotics such as chloramphenicol, erythromycin, and certain aminoglycosides and fluoroquinolones. Overexpression results in isopropyl-beta-D-thiogalactopyranoside (IPTG) exclusion and spectinomycin sensitivity. Transport of neutral substrates is electrogenic, whereas transport of cationic substrates is electroneutral. In addition to its role in multidrug resistance, confers extreme alkaline pH resistance, allowing the growth under conditions that are close to those used normally by alkaliphiles. This activity requires Na(+) or K(+).<ref>PMID:12578981</ref> <ref>PMID:15371593</ref> <ref>PMID:9079913</ref> <ref>PMID:9644262</ref> <ref>PMID:9811673</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The active efflux of antibiotics by multidrug-resistance (MDR) transporters is a major pathway of drug resistance and complicates the clinical treatment of bacterial infections. MdfA is a member of the major facilitator superfamily (MFS) from Escherichia coli and provides resistance to a wide variety of dissimilar toxic compounds, including neutral, cationic and zwitterionic substances. The 12-transmembrane-helix MdfA was expressed as a GFP-octahistidine fusion protein with a TEV protease cleavage site. Following tag removal, MdfA was purified using two chromatographic steps, complexed with a Fab fragment and further purified using size-exclusion chromatography. MdfA and MdfA-Fab complexes were subjected to both vapour-diffusion and lipidic cubic phase (LCP) crystallization techniques. Vapour-diffusion-grown crystals were of type II, with poor diffraction behaviour and weak crystal contacts. LCP lipid screening resulted in type I crystals that diffracted to 3.4 A resolution and belonged to the hexagonal space group P6122. | ||
| - | + | The multidrug-resistance transporter MdfA from Escherichia coli: crystallization and X-ray diffraction analysis.,Nagarathinam K, Jaenecke F, Nakada-Nakura Y, Hotta Y, Liu K, Iwata S, Stubbs MT, Nomura N, Tanabe M Acta Crystallogr F Struct Biol Commun. 2017 Jul 1;73(Pt 7):423-430. doi:, 10.1107/S2053230X17008500. Epub 2017 Jun 20. PMID:28695852<ref>PMID:28695852</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6gv1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Nagarathinam, K]] | ||
| + | [[Category: Parthier, C]] | ||
| + | [[Category: Stubbs, M T]] | ||
| + | [[Category: Tanabe, M]] | ||
| + | [[Category: Drug proton antiporter]] | ||
| + | [[Category: Major facilitator superfamily]] | ||
| + | [[Category: Mfs transporter]] | ||
| + | [[Category: Multidrug resistance]] | ||
| + | [[Category: Proton transport]] | ||
| + | [[Category: Transport protein]] | ||
Revision as of 07:54, 3 October 2018
Crystal structure of E.coli Multidrug/H+ antiporter MdfA in outward open conformation with bound Fab fragment
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