2btj

From Proteopedia

Revision as of 15:43, 5 November 2007

FLUORESCENT PROTEIN EOSFP- RED FORM

Overview

Genetically encoded fusion constructs derived from fluorescent proteins, (FPs) can be designed to report on a multitude of events and signals in, cells, tissues, and entire organs without interfering with the complex, machinery of life. EosFP is a novel FP from the scleractinian coral, Lobophyllia hemprichii that switches its fluorescence emission from green, (516 nm) to red (581 nm) upon irradiation with approximately 400-nm light., This property enables localized tagging of proteins and thus provides a, valuable tool for tracking protein movements within live cells. Here, we, present the x-ray structures of the green and red forms of WT EosFP. They, reveal that formation of the red chromophore is associated with cleavage, of the peptide backbone, with surprisingly little change elsewhere in the, structure, and provide insights into the mechanism that generates this, interesting posttranslational polypeptide modification.

About this Structure

2BTJ is a Single protein structure of sequence from Lobophyllia hemprichii with IEY as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP., Nienhaus K, Nienhaus GU, Wiedenmann J, Nar H, Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9156-9. Epub 2005 Jun 17. PMID:15964985

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