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5zwl

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Revision as of 08:38, 3 October 2018

Crystal structure of the gamma - epsilon complex of photosynthetic cyanobacterial F1-ATPase

Structural highlights

5zwl is a 2 chain structure with sequence from Theeb. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	atpC, atpE, tlr0526 (THEEB), atpG, atpC, tll0385 (THEEB)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ATPE_THEEB] Produces ATP from ADP in the presence of a proton gradient across the membrane.[HAMAP-Rule:MF_00530] The complex from the organism is particularly stable to disruption and remains functional after 6 hrs at 55 degrees Celsius.[HAMAP-Rule:MF_00530] [ATPG_THEEB] Produces ATP from ADP in the presence of a proton gradient across the membrane. The gamma chain is believed to be important in regulating ATPase activity and the flow of protons through the CF(0) complex. The complex from the organism is particularly stable to disruption and remains functional after 6 hrs at 55 degrees Celsius.^[1]

Publication Abstract from PubMed

F1-ATPase forms the membrane-associated segment of F0F1-ATP synthase - the fundamental enzyme complex in cellular bioenergetics for ATP hydrolysis and synthesis. Here, we report a crystal structure of the central F1 subcomplex, consisting of the rotary shaft gamma subunit and the inhibitory epsilon subunit, from the photosynthetic cyanobacterium Thermosynechococcus elongatus BP-1, at 1.98 A resolution. In contrast with their homologous bacterial and mitochondrial counterparts, the gamma subunits of photosynthetic organisms harbour a unique insertion of 35-40 amino acids. Our structural data reveal that this region forms a beta-hairpin structure along the central stalk. We identified numerous critical hydrogen bonds and electrostatic interactions between residues in the hairpin and the rest of the gamma subunit. To elaborate the critical function of this beta-hairpin in inhibiting ATP hydrolysis, the corresponding domain was deleted in the cyanobacterial F1 subcomplex. Biochemical analyses of the corresponding alpha3beta3gamma complex confirm that the clinch of the hairpin structure plays a critical role and accounts for a significant interaction in the alpha3beta3 complex to induce ADP inhibition during ATP hydrolysis. In addition, we found that truncating the beta-hairpin insertion structure resulted in a marked impairment of the interaction with the epsilon subunit, which binds to the opposite side of the gamma subunit from the beta-hairpin structure. Combined with structural analyses, our work provides experimental evidence supporting the molecular principle of how the insertion region of the gamma subunit suppresses F1 rotation during ATP hydrolysis.

Structure of the gamma-epsilon complex of cyanobacterial F1-ATPase reveals a suppression mechanism of the gamma subunit on ATP hydrolysis in phototrophs.,Murakami S, Kondo K, Katayama S, Hara S, Sunamura EI, Yamashita E, Groth G, Hisabori T Biochem J. 2018 Sep 18;475(18):2925-2939. doi: 10.1042/BCJ20180481. PMID:30054433^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Suhai T, Dencher NA, Poetsch A, Seelert H. Remarkable stability of the proton translocating F1FO-ATP synthase from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Biochim Biophys Acta. 2008 Apr;1778(4):1131-40. Epub 2007 Dec 31. PMID:18206981 doi:http://dx.doi.org/S0005-2736(07)00467-1
↑ Murakami S, Kondo K, Katayama S, Hara S, Sunamura EI, Yamashita E, Groth G, Hisabori T. Structure of the gamma-epsilon complex of cyanobacterial F1-ATPase reveals a suppression mechanism of the gamma subunit on ATP hydrolysis in phototrophs. Biochem J. 2018 Sep 18;475(18):2925-2939. doi: 10.1042/BCJ20180481. PMID:30054433 doi:http://dx.doi.org/10.1042/BCJ20180481

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zwl"

@@ Line 3: / Line 3: @@
 <StructureSection load='5zwl' size='340' side='right' caption='[[5zwl]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zwl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZWL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zwl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Theeb Theeb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZWL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZWL FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zwl OCA], [http://pdbe.org/5zwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zwl RCSB], [http://www.ebi.ac.uk/pdbsum/5zwl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zwl ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">atpC, atpE, tlr0526 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB]), atpG, atpC, tll0385 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197221 THEEB])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zwl OCA], [http://pdbe.org/5zwl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zwl RCSB], [http://www.ebi.ac.uk/pdbsum/5zwl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zwl ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Theeb]]
 [[Category: Hisabori, T]]
 [[Category: Murakami, S]]

5zwl

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Revision as of 08:38, 3 October 2018

Crystal structure of the gamma - epsilon complex of photosynthetic cyanobacterial F1-ATPase

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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