6cfx
From Proteopedia
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<StructureSection load='6cfx' size='340' side='right' caption='[[6cfx]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='6cfx' size='340' side='right' caption='[[6cfx]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6cfx]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CFX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6cfx]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bosea_sp._root381 Bosea sp. root381]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CFX FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ASE63_04290 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1736524 Bosea sp. Root381])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cfx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfx OCA], [http://pdbe.org/6cfx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cfx RCSB], [http://www.ebi.ac.uk/pdbsum/6cfx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfx ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cfx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfx OCA], [http://pdbe.org/6cfx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cfx RCSB], [http://www.ebi.ac.uk/pdbsum/6cfx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfx ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | When DNA is unwound during replication, it becomes overtwisted and forms positive supercoils in front of the translocating DNA polymerase. Unless removed or dissipated, this superhelical tension can impede replication elongation. Topoisomerases, including gyrase and topoisomerase IV in bacteria, are required to relax positive supercoils ahead of DNA polymerase but may not be sufficient for replication. Here, we find that GapR, a chromosome structuring protein in Caulobacter crescentus, is required to complete DNA replication. GapR associates in vivo with positively supercoiled chromosomal DNA, and our biochemical and structural studies demonstrate that GapR forms a dimer-of-dimers that fully encircles overtwisted DNA. Further, we show that GapR stimulates gyrase and topo IV to relax positive supercoils, thereby enabling DNA replication. Analogous chromosome structuring proteins that locate to the overtwisted DNA in front of replication forks may be present in other organisms, similarly helping to recruit and stimulate topoisomerases during DNA replication. | ||
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| + | A Bacterial Chromosome Structuring Protein Binds Overtwisted DNA to Stimulate Type II Topoisomerases and Enable DNA Replication.,Guo MS, Haakonsen DL, Zeng W, Schumacher MA, Laub MT Cell. 2018 Sep 7. pii: S0092-8674(18)31045-6. doi: 10.1016/j.cell.2018.08.029. PMID:30220456<ref>PMID:30220456</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6cfx" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Bosea sp. root381]] | ||
[[Category: Schumacher, M A]] | [[Category: Schumacher, M A]] | ||
[[Category: Caulobacter]] | [[Category: Caulobacter]] | ||
Revision as of 08:42, 3 October 2018
Bosea sp GapR solved in the presence of DNA
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