2qvw
From Proteopedia
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|PDB= 2qvw |SIZE=350|CAPTION= <scene name='initialview01'>2qvw</scene>, resolution 3.000Å | |PDB= 2qvw |SIZE=350|CAPTION= <scene name='initialview01'>2qvw</scene>, resolution 3.000Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2ffl|2FFL]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qvw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvw OCA], [http://www.ebi.ac.uk/pdbsum/2qvw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qvw RCSB]</span> | ||
}} | }} | ||
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[[Category: Doudna, J A.]] | [[Category: Doudna, J A.]] | ||
[[Category: MacRae, I J]] | [[Category: MacRae, I J]] | ||
| - | [[Category: MN]] | ||
[[Category: dicer]] | [[Category: dicer]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | [[Category: pseudo-merohedral twin | + | [[Category: rnai,pseudo-merohedral twin]] |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:53:56 2008'' |
Revision as of 01:53, 31 March 2008
| |||||||
| , resolution 3.000Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Ribonuclease III, with EC number 3.1.26.3 | ||||||
| Related: | 2FFL
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Giardia Dicer refined against twinned data
Overview
The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.
About this Structure
2QVW is a Single protein structure of sequence from Giardia intestinalis. Full crystallographic information is available from OCA.
Reference
An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:17704568
Page seeded by OCA on Mon Mar 31 04:53:56 2008
