2qxh

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|PDB= 2qxh |SIZE=350|CAPTION= <scene name='initialview01'>2qxh</scene>, resolution 2.00&Aring;
|PDB= 2qxh |SIZE=350|CAPTION= <scene name='initialview01'>2qxh</scene>, resolution 2.00&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=K7J:'>K7J</scene>
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|LIGAND= <scene name='pdbligand=K7J:N-(3-CARBOXYPROPANOYL)-L-ALANYL-L-ALANYL-N-[(1S,2R)-1-BENZYL-2-HYDROXYPROPYL]-L-PROLINAMIDE'>K7J</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Stratum_corneum_chymotryptic_enzyme Stratum corneum chymotryptic enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.117 3.4.21.117]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Stratum_corneum_chymotryptic_enzyme Stratum corneum chymotryptic enzyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.117 3.4.21.117] </span>
|GENE= KLK7, PRSS6, SCCE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
|GENE= KLK7, PRSS6, SCCE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
 +
|DOMAIN=
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|RELATEDENTRY=[[2qxg|2QXG]], [[2qxi|2QXI]], [[2qxj|2QXJ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qxh OCA], [http://www.ebi.ac.uk/pdbsum/2qxh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qxh RCSB]</span>
}}
}}
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[[Category: Magdolen, V.]]
[[Category: Magdolen, V.]]
[[Category: Schechter, N M.]]
[[Category: Schechter, N M.]]
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[[Category: K7J]]
 
[[Category: 70-80 loop]]
[[Category: 70-80 loop]]
[[Category: active site inhibitor]]
[[Category: active site inhibitor]]
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[[Category: zymogen]]
[[Category: zymogen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:30:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:54:26 2008''

Revision as of 01:54, 31 March 2008

Image:2qxh.jpg


PDB ID 2qxh

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands:
Gene: KLK7, PRSS6, SCCE (Homo sapiens)
Activity: Stratum corneum chymotryptic enzyme, with EC number 3.4.21.117
Related: 2QXG, 2QXI, 2QXJ


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Human Kallikrein 7 in Complex with Suc-Ala-Ala-Pro-Phe-chloromethylketone


Overview

hK7 or human stratum corneum chymotryptic enzyme belongs to the human tissue kallikrein (hKs) serine proteinase family and is strongly expressed in the upper layers of the epidermis. It participates in skin desquamation but is also implicated in diverse skin diseases and is a potential biomarker of ovarian cancer. We have solved x-ray structures of recombinant active hK7 at medium and atomic resolution in the presence of the inhibitors succinyl-Ala-Ala-Pro-Phe-chloromethyl ketone and Ala-Ala-Phe-chloromethyl ketone. The most distinguishing features of hK7 are the short 70-80 loop and the unique S1 pocket, which prefers P1 Tyr residues, as shown by kinetic data. Similar to several other kallikreins, the enzyme activity is inhibited by Zn(2+) and Cu(2+) at low micromolar concentrations. Biochemical analyses of the mutants H99A and H41F confirm that only the metal-binding site at His(99) close to the catalytic triad accounts for the noncompetitive Zn(2+) inhibition type. Additionally, hK7 exhibits large positively charged surface patches, representing putative exosites for prime side substrate recognition.

About this Structure

2QXH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Chymotryptic specificity determinants in the 1.0 A structure of the zinc-inhibited human tissue kallikrein 7., Debela M, Hess P, Magdolen V, Schechter NM, Steiner T, Huber R, Bode W, Goettig P, Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16086-91. Epub 2007 Oct 1. PMID:17909180

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