2r5o
From Proteopedia
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|PDB= 2r5o |SIZE=350|CAPTION= <scene name='initialview01'>2r5o</scene>, resolution 1.300Å | |PDB= 2r5o |SIZE=350|CAPTION= <scene name='initialview01'>2r5o</scene>, resolution 1.300Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= ORF431 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= ORF431 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5o OCA], [http://www.ebi.ac.uk/pdbsum/2r5o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r5o RCSB]</span> | ||
}} | }} | ||
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[[Category: Kimber, M S.]] | [[Category: Kimber, M S.]] | ||
[[Category: Whitfield, C.]] | [[Category: Whitfield, C.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: NA]] | ||
| - | [[Category: PG4]] | ||
[[Category: abc transporter]] | [[Category: abc transporter]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
| Line 37: | Line 37: | ||
[[Category: transport protein]] | [[Category: transport protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:56:49 2008'' |
Revision as of 01:56, 31 March 2008
| |||||||
| , resolution 1.300Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | ORF431 (Escherichia coli) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the C-terminal domain of wzt
Overview
ATP-binding-cassette (ABC) transporters are responsible for the export of a wide variety of cell-surface glycoconjugates in both Gram-positive and Gram-negative bacteria. These include the O-antigenic polysaccharide (O-PS) portion of lipopolysaccharide, a crucial virulence determinant in Gram-negative pathogens. O-PSs are synthesized by one of two fundamentally different pathways. Escherichia coli O serotypes O8 and O9a provide the prototype systems for studying O-PS export via ABC transporters. The transporter is composed of the transmembrane component Wzm and the nucleotide-binding component Wzt. Although the N-terminal domain of Wzt is a conventional ABC protein, the C-terminal domain of Wzt (C-Wzt) is a unique structural element that determines the specificity of the transporter for either the O8 or O9a O-PS. We show here that the two domains of Wzt can function when expressed as separate polypeptides; both are essential for export. In vitro, C-Wzt binds its cognate O-PS by recognizing a residue located at the nonreducing end of the polymer. The crystal structure of C-Wzt(O9a) is reported here and reveals a beta sandwich with an immunoglobulin-like topology that contains the O-PS-binding pocket. Substrate interactions with nucleotide-binding domains have been demonstrated in an ABC exporter previously. However, to our knowledge substrate binding by a discrete, cytoplasmic accessory domain in an extended nucleotide-binding domain polypeptide has not previously been demonstrated. Elucidation of the substrate-recognition system involved in O-PS export provides insight into the mechanism that coordinates polymer biosynthesis, termination, and export.
About this Structure
2R5O is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Substrate binding by a bacterial ABC transporter involved in polysaccharide export., Cuthbertson L, Kimber MS, Whitfield C, Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19529-34. Epub 2007 Nov 21. PMID:18032609
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