2r9a
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2r9a |SIZE=350|CAPTION= <scene name='initialview01'>2r9a</scene>, resolution 2.50Å | |PDB= 2r9a |SIZE=350|CAPTION= <scene name='initialview01'>2r9a</scene>, resolution 2.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= NHEJ1, XLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= NHEJ1, XLF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r9a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r9a OCA], [http://www.ebi.ac.uk/pdbsum/2r9a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2r9a RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Junop, M S.]] | [[Category: Junop, M S.]] | ||
[[Category: alternative splicing]] | [[Category: alternative splicing]] | ||
| - | [[Category: | + | [[Category: cernunnos,non-homologous end joining]] |
[[Category: disease mutation]] | [[Category: disease mutation]] | ||
[[Category: dna damage]] | [[Category: dna damage]] | ||
[[Category: dna double strand break repair]] | [[Category: dna double strand break repair]] | ||
[[Category: dna repair]] | [[Category: dna repair]] | ||
| - | [[Category: non-homologous end joining]] | ||
[[Category: nucleus]] | [[Category: nucleus]] | ||
[[Category: protein binding]] | [[Category: protein binding]] | ||
[[Category: xlf]] | [[Category: xlf]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:57:46 2008'' |
Revision as of 01:57, 31 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | NHEJ1, XLF (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of human XLF
Overview
DNA double-strand breaks represent one of the most severe forms of DNA damage in mammalian cells. One pathway for repairing these breaks occurs via nonhomologous end-joining (NHEJ) and depends on XRCC4, LigaseIV, and Cernunnos, also called XLF. Although XLF stimulates XRCC4/LigaseIV to ligate mismatched and noncohesive DNA ends, the mechanistic basis for this function remains unclear. Here we report the structure of a partially functional 224 residue N-terminal fragment of human XLF. Despite only weak sequence similarity, XLF(1-170) shares structural homology with XRCC4(1-159). However, unlike the highly extended 130 A helical domain observed in XRCC4, XLF adopts a more compact, folded helical C-terminal region involving two turns and a twist, wrapping back to the structurally conserved N terminus. Mutational analysis of XLF and XRCC4 reveals a potential interaction interface, suggesting a mechanism for how XLF stimulates the ligation of mismatched ends.
About this Structure
2R9A is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human XLF: a twist in nonhomologous DNA end-joining., Andres SN, Modesti M, Tsai CJ, Chu G, Junop MS, Mol Cell. 2007 Dec 28;28(6):1093-101. PMID:18158905
Page seeded by OCA on Mon Mar 31 04:57:46 2008
