2rf4

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|PDB= 2rf4 |SIZE=350|CAPTION= <scene name='initialview01'>2rf4</scene>, resolution 3.1&Aring;
|PDB= 2rf4 |SIZE=350|CAPTION= <scene name='initialview01'>2rf4</scene>, resolution 3.1&Aring;
|SITE=
|SITE=
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|LIGAND=
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
|GENE= RPA43, RRN12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RPA14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
|GENE= RPA43, RRN12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RPA14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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|DOMAIN=
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|RELATEDENTRY=[[1y14|1Y14]], [[2ckz|2CKZ]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rf4 OCA], [http://www.ebi.ac.uk/pdbsum/2rf4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rf4 RCSB]</span>
}}
}}
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[[Category: transferase dna/rna]]
[[Category: transferase dna/rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:35:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:59:41 2008''

Revision as of 01:59, 31 March 2008

Image:2rf4.jpg


PDB ID 2rf4

Drag the structure with the mouse to rotate
, resolution 3.1Å
Ligands:
Gene: RPA43, RRN12 (Saccharomyces cerevisiae), RPA14 (Saccharomyces cerevisiae)
Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6
Related: 1Y14, 2CKZ


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the RNA Polymerase I subcomplex A14/43


Overview

Synthesis of ribosomal RNA (rRNA) by RNA polymerase (Pol) I is the first step in ribosome biogenesis and a regulatory switch in eukaryotic cell growth. Here we report the 12 A cryo-electron microscopic structure for the complete 14-subunit yeast Pol I, a homology model for the core enzyme, and the crystal structure of the subcomplex A14/43. In the resulting hybrid structure of Pol I, A14/43, the clamp, and the dock domain contribute to a unique surface interacting with promoter-specific initiation factors. The Pol I-specific subunits A49 and A34.5 form a heterodimer near the enzyme funnel that acts as a built-in elongation factor and is related to the Pol II-associated factor TFIIF. In contrast to Pol II, Pol I has a strong intrinsic 3'-RNA cleavage activity, which requires the C-terminal domain of subunit A12.2 and, apparently, enables ribosomal RNA proofreading and 3'-end trimming.

About this Structure

2RF4 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Functional architecture of RNA polymerase I., Kuhn CD, Geiger SR, Baumli S, Gartmann M, Gerber J, Jennebach S, Mielke T, Tschochner H, Beckmann R, Cramer P, Cell. 2007 Dec 28;131(7):1260-72. PMID:18160037

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