2zoy

From Proteopedia

(Difference between revisions)

Revision as of 07:32, 18 October 2018

The multi-drug binding transcriptional repressor CgmR (CGL2612 protein) from C.glutamicum

Structural highlights

2zoy is a 2 chain structure with sequence from Corgl. This structure supersedes the now removed PDB entry 1v7b. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2zoz, 2yve, 2yvh
Gene:	Cgl2612 (CORGL)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The emergence of antibiotic-resistant bacteria often causes serious clinical problems. The TetR family is one of the major transcription factor families that regulate expression of genes involved in bacterial antimicrobial resistance systems. CGL2612 protein is a transcription factor newly identified by genomic DNA analysis on Corynebacterium glutamicum, which belongs to the mycolic acid-containing Actinomycetales, including the well known pathogens Corynebacterium diphtheriae and Mycobacterium tuberculosis. Crystal structure analysis showed that the CGL2612 protein exhibits significant structural similarity to the multidrug resistance (MDR)-related transcription factor QacR from Staphylococcus aureus, despite poor amino acid sequence similarity between these proteins. Binding DNA sequence analysis of CGL2612 protein using the systematic evolution of ligands by the exponential enrichment (systematic evolution of ligands by exponential enrichment, or SELEX) method revealed that this protein is a new member of the TetR family, which regulates expression of the immediately upstream gene, cgl2611, probably encoding a major facilitator superfamily permease. Subsequent functional analyses confirmed a function of the CGL2612 as a transcriptional repressor responsible for the antimicrobial resistance system in C. glutamicum. The strategy used in the present study is one of the most convenient and powerful methods to analyze functionally unknown transcription factors, and the results obtained here will contribute to our understanding of the drug resistance mechanism not only in C. glutamicum but also in the related bacteria, C. diphtheriae and M. tuberculosis.

The CGL2612 protein from Corynebacterium glutamicum is a drug resistance-related transcriptional repressor: structural and functional analysis of a newly identified transcription factor from genomic DNA analysis.,Itou H, Okada U, Suzuki H, Yao M, Wachi M, Watanabe N, Tanaka I J Biol Chem. 2005 Nov 18;280(46):38711-9. Epub 2005 Sep 15. PMID:16166084^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Itou H, Okada U, Suzuki H, Yao M, Wachi M, Watanabe N, Tanaka I. The CGL2612 protein from Corynebacterium glutamicum is a drug resistance-related transcriptional repressor: structural and functional analysis of a newly identified transcription factor from genomic DNA analysis. J Biol Chem. 2005 Nov 18;280(46):38711-9. Epub 2005 Sep 15. PMID:16166084 doi:http://dx.doi.org/10.1074/jbc.M505999200

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2zoy"

@@ Line 1: / Line 1: @@
 ==The multi-drug binding transcriptional repressor CgmR (CGL2612 protein) from C.glutamicum==
 <StructureSection load='2zoy' size='340' side='right' caption='[[2zoy]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2zoz|2zoz]], [[2yve|2yve]], [[2yvh|2yvh]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cgl2612 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196627 CORGL])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zoy OCA], [http://pdbe.org/2zoy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zoy RCSB], [http://www.ebi.ac.uk/pdbsum/2zoy PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zoy OCA], [http://pdbe.org/2zoy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zoy RCSB], [http://www.ebi.ac.uk/pdbsum/2zoy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zoy ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 12: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zo/2zoy_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zo/2zoy_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2zoy

From Proteopedia

Revision as of 07:32, 18 October 2018

The multi-drug binding transcriptional repressor CgmR (CGL2612 protein) from C.glutamicum

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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