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2rmj

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(New page: 200px {{Structure |PDB= 2rmj |SIZE=350|CAPTION= <scene name='initialview01'>2rmj</scene> |SITE= |LIGAND= |ACTIVITY= |GENE= DDX58 ([http://www.ncbi.nlm.nih.gov/...)
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|GENE= DDX58 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
|GENE= DDX58 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rmj OCA], [http://www.ebi.ac.uk/pdbsum/2rmj PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2rmj RCSB]</span>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rmj OCA], [http://www.ebi.ac.uk/pdbsum/2rmj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rmj RCSB]</span>
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[[Category: ubl conjugation]]
[[Category: ubl conjugation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:02:02 2008''

Revision as of 02:02, 31 March 2008


PDB ID 2rmj

Drag the structure with the mouse to rotate
Gene: DDX58 (Homo sapiens)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Solution structure of RIG-I C-terminal domain


Overview

A DExD/H protein, RIG-I, is critical in innate antiviral responses by sensing viral RNA. Here we show that RIG-I recognizes two distinct viral RNA patterns: double-stranded (ds) and 5'ppp single-stranded (ss) RNA. The binding of RIG-I with dsRNA or 5'ppp ssRNA in the presence of ATP produces a common structure, as suggested by protease digestion. Further analyses demonstrated that the C-terminal domain of RIG-I (CTD) recognizes these RNA patterns and CTD coincides with the autorepression domain. Structural analysis of CTD by NMR spectroscopy in conjunction with mutagenesis revealed that the basic surface of CTD with a characteristic cleft interacts with RIG-I ligands. Our results suggest that the bipartite structure of CTD regulates RIG-I on encountering viral RNA patterns.

About this Structure

2RMJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Nonself RNA-sensing mechanism of RIG-I helicase and activation of antiviral immune responses., Takahasi K, Yoneyama M, Nishihori T, Hirai R, Kumeta H, Narita R, Gale M Jr, Inagaki F, Fujita T, Mol Cell. 2008 Feb 29;29(4):428-40. Epub 2008 Jan 31. PMID:18242112

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